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Title:	A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
Author:	Colmán Martínez, Lourdes Mariel; Martínez Huélamo, Miriam; Miralles, Esther; Estruch Riba, Ramon; Lamuela Raventós, Rosa Ma.
Notes:	A simple and accurate reversed phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) method for simultaneously determining and quantifying the antioxidants carotenes, xanthophylls, and retinol in human plasma is presented in this paper. Compounds were extracted with hexane, a C30 column, and a mobile phase of methanol, methyl tert-butyl ether, and water were used for the separation of the compounds. A total of 8 carotenoids, 3 Z--carotene isomers, and 1 fat-soluble vitamin (retinol) were resolved within 72 min at a flow rate of 0.6 mL/min. Detection was achieved at 450 nm for carotenoids and 330 nm for retinol. To evaluate the effectiveness of themethod, it has been applied to an intervention study conducted on eight volunteers. Results. Limits of detection were between 0.1 g/mL for lycopene and astaxanthin and 1.3 g/mL for 15-Z--carotene. Recoveries were ranged between 89% and 113% for -carotene and astaxanthin, respectively. Accuracy was between 90.7% and 112.2% and precision was between 1% and 15% RSD. In human plasma samples compounds studied were identified besides three lycopene isomers, demonstrated to be suitable for application in dietary intervention studies. Conclusions. Due to its accuracy, precision, selectivity, and reproducibility, this method is suitable to dietary habits and/or antioxidants status studies.
Subject(s):	-Cromatografia de líquids -Antioxidants -Carotenoides -Vitamina A -Plasma sanguini -Liquid chromatography -Antioxidants -Carotenoids -Vitamin A -Blood plasma
Rights:	cc-by (c) Colmán Martínez, Lourdes Mariel et al., 2015 http://creativecommons.org/licenses/by/3.0/es
Document type:	Article Article - Published version
Published by:	Hindawi
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