Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/117815
| dc.contributor.author | Martínez Barriocanal, Águeda |
|---|---|
| dc.contributor.author | Arcas García, Andrea |
| dc.contributor.author | Magallon Lorenz, Miriam |
| dc.contributor.author | Ejarque Ortiz, Aroa |
| dc.contributor.author | Negro Demontel, M. Luciana |
| dc.contributor.author | Comas Casellas, Emma |
| dc.contributor.author | Schwartz Navarro, Simó |
| dc.contributor.author | Malhotra, Sunny |
| dc.contributor.author | Montalbán Gairín, Xavier |
| dc.contributor.author | Peluffo, Hugo |
| dc.contributor.author | Martín Andorrà, Margarita |
| dc.contributor.author | Comabella, Manuel |
| dc.contributor.author | Sayós Ortega, Juan |
| dc.date | 2017-11-15T16:58:40Z |
| dc.date | 2017-11-15T16:58:40Z |
| dc.date | 2017-10-19 |
| dc.date | 2017-11-15T16:58:41Z |
| dc.identifier | 2045-2322 |
| dc.identifier | 674150 |
| dc.identifier | 29051512 |
| dc.identifier.uri | http://hdl.handle.net/2445/117815 |
| dc.description | Herein, we have used bioinformatics tools to predict five clusters defining ligand-binding sites on the extracellular domain of human CD300b receptor, presumably involved in the formation of both homodimers and heterodimers with other CD300 family members. Site-directed mutagenesis revealed residues glutamic acid 28 and glutamine 29 in cluster 5 to be necessary for the formation of CD300b complexes. Surprisingly, the disruption of cluster 2 and 4 reconstituted the binding capability lost by the mutation of residues glutamic acid 28 to alanine, glutamine 29 to alanine (E28A-Q29G). We identified a missense mutation arginine 33 to glutamine (R33Q) in CD300f by direct sequencing of exon 2 in peripheral blood samples from 50 patients with multiple sclerosis (MS). Levels of expression of CD300f were almost undetectable on monocytes from the patient bearing the R33Q mutation compared with healthy individuals. Whereas R33Q mutation had no effect in the formation of CD300f complexes, the inhibition of protein synthesis with cycloheximide indicated that CD300f R33Q is less stable than native CD300f. Finally, we report that the levels of expression of CD300f on the surface of classical and intermediate monocytes from MS patients are significantly lower when compared to the same cell populations in healthy individuals. |
| dc.format | 11 p. |
| dc.format | application/pdf |
| dc.language | eng |
| dc.publisher | Nature Publishing Group |
| dc.relation | Reproducció del document publicat a: https://doi.org/10.1038/s41598-017-12881-8 |
| dc.relation | Scientific Reports, 2017, vol. 7, num. 13544 |
| dc.relation | https://doi.org/10.1038/s41598-017-12881-8 |
| dc.rights | cc-by (c) Martínez Barriocanal, Águeda et al., 2017 |
| dc.rights | http://creativecommons.org/licenses/by/3.0/es |
| dc.rights | info:eu-repo/semantics/openAccess |
| dc.subject | Esclerosi múltiple |
| dc.subject | Mutació (Biologia) |
| dc.subject | Receptors cel·lulars |
| dc.subject | Multiple sclerosis |
| dc.subject | Mutation (Biology) |
| dc.subject | Cell receptors |
| dc.title | Effect of Specific Mutations in Cd300 Complexes Formation; Potential Implication of Cd300f in Multiple Sclerosis. |
| dc.type | info:eu-repo/semantics/article |
| dc.type | info:eu-repo/semantics/publishedVersion |
Documentos relacionados
Otros documentos del mismo autor/a
Martínez Barriocanal, Águeda; Arcas García, Andrea; Magallon Lorenz, Miriam; Ejarque Ortiz, Aroa; Negro Demontel, M.Luciana; Comas Casellas, Emma; Schwartz Navarro, Simó; Malhotra, Sunny; Montalbán Gairín, Xavier; Peluffo, Hugo; Martín Andorrà, Margarita; Comabella López, Manuel; Sayós Ortega, Joan
Ejarque Ortiz, Aroa; Solà, C.; Martínez Barriocanal, Águeda; Schwartz Navarro, Simó; Martín Andorrà, Margarita; Peluffo, Hugo; Sayós Ortega, Joan
Malhotra, Sunny; Villar, Luisa M.; Costa, Carme; Midaglia, Luciana; Cubedo Culleré, Marta; Medina Casanellas, Sílvia; Fissolo, Nicolás; Río, Jordi; Castilló, Joaquín; Álvarez Cermeño, José C.; Sànchez, Àlex (Sànchez Pla); Montalbán Gairín, Xavier; Comabella, Manuel
Serrano Candelas, Eva; Ainsua-Enrich, Erola; Navinés Ferrer, Arnau; Rodrigues, Paulo; García Valverde, Alfonso; Bazzocco, Sarah; Macaya, Irati; Arribas López, Joaquín; Serrano, César; Sayós Ortega, Juan; Arango del Corro, Diego; Martín Andorrà, Margarita
Malhotra, Sunny; Bustamante, Marta F.; Perez Miralles, Francisco; Rio, Jordi; Ruiz de Villa, Carmen; Vegas, Esteban; Nonell, Lara; Deisenhammer, Florian; Fissolo, Nicolás.; Nurtdinov, Ramil N.; Montalbán Gairín, Xavier; Comabella López, Manuel