To access the full text documents, please follow this link: http://hdl.handle.net/2445/107636
| dc.contributor.author | Orta Mascaró, Marc |
|---|---|
| dc.contributor.author | Consuegra Fernández, Marta |
| dc.contributor.author | Carreras Margalef, Esther |
| dc.contributor.author | Roncagalli, Romain |
| dc.contributor.author | Carreras Sureda, Amado |
| dc.contributor.author | Alvarez, Pilar |
| dc.contributor.author | Girard, Laura |
| dc.contributor.author | Simões, Inês |
| dc.contributor.author | Martínez-Florensa, Mario |
| dc.contributor.author | Aranda, Fernando |
| dc.contributor.author | Merino, Ramón |
| dc.contributor.author | Martinez, Vanesa Gabriela |
| dc.contributor.author | Vicente García, Rubén, 1978- |
| dc.contributor.author | Merino, Jesús |
| dc.contributor.author | Sarukhan, Adelaida |
| dc.contributor.author | Malissen, Marie |
| dc.contributor.author | Malissen, Bernard |
| dc.contributor.author | Lozano Soto, Francisco |
| dc.date | 2017-03-01T15:47:43Z |
| dc.date | 2017-07-25T22:01:25Z |
| dc.date | 2016-07-04 |
| dc.date | 2017-03-01T15:47:43Z |
| dc.identifier | 0896-548X |
| dc.identifier | 667668 |
| dc.identifier | 27377588 |
| dc.identifier.uri | http://hdl.handle.net/2445/107636 |
| dc.description | The CD6 glycoprotein is a lymphocyte surface receptor putatively involved in T cell development and activation. CD6 facilitates adhesion between T cells and antigen-presenting cells through its interaction with CD166/ALCAM (activated leukocyte cell adhesion molecule), and physically associates with the T cell receptor (TCR) at the center of the immunological synapse. However, its precise role during thymocyte development and peripheral T cell immune responses remains to be defined. Here, we analyze the in vivo consequences of CD6 deficiency. CD6(-/-) thymi showed a reduction in both CD4(+) and CD8(+) single-positive subsets, and double-positive thymocytes exhibited increased Ca(2+) mobilization to TCR cross-linking in vitro. Bone marrow chimera experiments revealed a T cell-autonomous selective disadvantage of CD6(-/-) T cells during development. The analysis of TCR-transgenic mice (OT-I and Marilyn) confirmed that abnormal T cell selection events occur in the absence of CD6. CD6(-/-) mice displayed increased frequencies of antigen-experienced peripheral T cells generated under certain levels of TCR signal strength or co-stimulation, such as effector/memory (CD4(+)TEM and CD8(+)TCM) and regulatory (T reg) T cells. The suppressive activity of CD6(-/-) T reg cells was diminished, and CD6(-/-) mice presented an exacerbated autoimmune response to collagen. Collectively, these data indicate that CD6 modulates the threshold for thymocyte selection and the generation and/or function of several peripheral T cell subpopulations, including T reg cells. |
| dc.format | 11 p. |
| dc.format | application/pdf |
| dc.language | eng |
| dc.publisher | Wiley |
| dc.relation | Versió postprint del document publicat a: https://doi.org/10.1084/jem.20151785 |
| dc.relation | The Journal of Trace Elements in Experimental Medicine, 2016, vol. 213, num. 8, p. 1387-1397 |
| dc.relation | https://doi.org/10.1084/jem.20151785 |
| dc.relation | info:eu-repo/grantAgreement/EC/FP7/229673/EU//BIOTRACK |
| dc.relation | info:eu-repo/grantAgreement/EC/FP7/322465/EU//INTEGRATE |
| dc.rights | (c) Wiley, 2016 |
| dc.rights | info:eu-repo/semantics/openAccess |
| dc.subject | Biologia molecular |
| dc.subject | Receptors cel·lulars |
| dc.subject | Homeòstasi |
| dc.subject | Cèl·lules T |
| dc.subject | Timus (Glàndula) |
| dc.subject | Molecular biology |
| dc.subject | Cell receptors |
| dc.subject | Homeostasis |
| dc.subject | T cells |
| dc.subject | Thymus |
| dc.title | CD6 modulates thymocyte selection and peripheral T cell homeostasis |
| dc.type | info:eu-repo/semantics/article |
| dc.type | info:eu-repo/semantics/acceptedVersion |
Related documents
Other documents of the same author
Orta Mascaró, Marc; Consuegra Fernández, Marta; Carreras Margalef, Esther; Roncagalli, Romain; Carreras Sureda, Amado; Alvarez, Pilar; Girard, Laura; Simões, Inês; Martínez-Florensa, Mario; Aranda, Fernando; Merino, Ramón; Martínez, Vanesa Gabriela; Vicente, Rubén; Merino, Jesús; Sarukhan, Adelaida; Malissen, Marie; Malissen, Bernard; Lozano Soto, Francisco
Fenutría, Rafael; Martinez, Vanesa Gabriela; Simões, Inês; Postigo, Jorge; Gil, Victor; Martínez-Florensa, Mario; Sintes, Jordi; Naves, Rodrigo; Cashman, Kevin S.; Alberola-Ila, José; Ramos Casals, Manuel; Soldevila, Gloria; Raman, Chander; Merino, Jesús; Merino, Ramón; Engel Rocamora, Pablo; Lozano Soto, Francisco
Carrasco, Esther; Escoda Ferran, Cristina; Climent, Núria; Miró Julià, Cristina; Simões, Inês; Martínez-Florensa, Mario; Sarukhan, Adelaida; Carreras Margalef, Esther; Lozano Soto, Francisco
Carrasco, Esther; Escoda Ferran, Cristina; Climent, Núria; Miró Julià, Cristina; Simões, Inês; Martínez-Florensa, Mario; Sarukhan, Adelaida; Carreras Margalef, Esther; Lozano Soto, Francisco
Fenutría, Rafael; Martinez, Vanesa G.; Simões, Inês; Postigo, Jorge; Gil, Victor; Martinez-Florensa, Mario; Sintes, Jordi; Naves, Rodrigo; Cashman, Kevin S.; Alberola-Ila, José; Ramos Casals, Manuel; Soldevila, Gloria; Raman, Chander; Merino, Jesús; Merino, Ramón; Engel Rocamora, Pablo; Lozano Soto, Francisco