dc.contributor.author |
Ancín, María |
dc.contributor.author |
Larraya, Luis |
dc.contributor.author |
Florez-Sarasa, Igor |
dc.contributor.author |
Bénard, Camille |
dc.contributor.author |
Fernández-San Millán, Alicia |
dc.contributor.author |
Veramendi, Jon |
dc.contributor.author |
Gibon, Yves |
dc.contributor.author |
Fernie, Alisdair |
dc.contributor.author |
Aranjuelo, Iker |
dc.contributor.author |
Farran, Inmaculada |
dc.date |
2021 |
dc.identifier |
https://ddd.uab.cat/record/255555 |
dc.identifier |
urn:10.1093/jxb/erab193 |
dc.identifier |
urn:oai:ddd.uab.cat:255555 |
dc.identifier |
urn:pmcid:PMC8219043 |
dc.identifier |
urn:pmc-uid:8219043 |
dc.identifier |
urn:pmid:33963398 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:8219043 |
dc.identifier |
urn:articleid:14602431v72n13p4949 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Ministerio de Ciencia e Innovación AGL2016-79868 |
dc.relation |
Ministerio de Educación, Cultura y Deporte FPU13/01675 |
dc.relation |
European Commission 753301 |
dc.relation |
Agencia Estatal de Investigación RYC2019-027244-I |
dc.relation |
Journal of experimental botany ; Vol. 72, Issue 13 (May 2021), p. 4949-4964 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Carbon metabolism |
dc.subject |
Chloroplast |
dc.subject |
Glutamine synthetase |
dc.subject |
GS-GOGAT pathway |
dc.subject |
Nitrogen metabolism |
dc.subject |
Photorespiration |
dc.subject |
Thioredoxin |
dc.title |
Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco |
dc.type |
Article |
dc.description.abstract |
In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts. |