Título:
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Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
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Autor/a:
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Ancín, María; Larraya, Luis; Florez-Sarasa, Igor; Bénard, Camille; Fernández-San Millán, Alicia; Veramendi, Jon; Gibon, Yves; Fernie, Alisdair; Aranjuelo, Iker; Farran, Inmaculada
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Abstract:
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In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts. |
Materia(s):
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-Carbon metabolism -Chloroplast -Glutamine synthetase -GS-GOGAT pathway -Nitrogen metabolism -Photorespiration -Thioredoxin |
Derechos:
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open access
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Tipo de documento:
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Article |
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Uri:
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https://ddd.uab.cat/record/255555
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