Institut de Recerca Biomèdica (IRB Barcelona): Recent submissions
Now showing items 81-100 of 418
The conserved lysine residue in transmembrane helix 5 is key for the cytoplasmic gating of the L-amino acid transporters
L-Amino acid transporters (LATs) play a key role in a wide range of physiological processes. Defects in LATs can lead to neurological disorders and aminoacidurias, while the overexpression of these transporters is related ...
RANK links senescence to stemness in the mammary epithelia, delaying tumor onset but increasing tumor aggressiveness
Rank signaling enhances stemness in mouse and human mammary epithelial cells (MECs) and mediates mammary tumor initiation. Mammary tumors initiated by oncogenes or carcinogen exposure display high levels of Rank and Rank ...
Senescence-associated morphological profiles (SAMPs): an image-based phenotypic profiling method for evaluating the inter and intra model heterogeneity of senescence
Senescence occurs in response to a number of damaging stimuli to limit oncogenic transformation and cancer development. As no single, universal senescence marker has been discovered, the confident classification of senescence ...
The metabolic co-regulator PGC1α suppresses prostate cancer metastasis
Cellular transformation and cancer progression is accompanied by changes in the metabolic landscape. Master co-regulators of metabolism orchestrate the modulation of multiple metabolic pathways through transcriptional ...
Zebrafish models for human acute organophosphorus poisoning
Terrorist use of organophosphorus-based nerve agents and toxic industrial chemicals against civilian populations constitutes a real threat, as demonstrated by the terrorist attacks in Japan in the 1990 s or, even more ...
Molecular dynamics modelling of the interaction of a synthetic zinc-finger miniprotein with DNA
We report the modelling of the DNA complex of an artificial miniprotein composed of two zinc finger modules and an AT-hook linking peptide. The computational study provides for the first time a structural view of these ...
BIGNASim: A NoSQL database structure and analysis portal for nucleic acids simulation data
Molecular dynamics simulation (MD) is, just behind genomics, the bioinformatics tool that generates the largest amounts of data, and that is using the largest amount of CPU time in supercomputing centres. MD trajectories ...
Sensitive high-throughput single-cell RNA-seq reveals within-clonal transcript correlations in yeast populations
Single-cell RNA sequencing has revealed extensive cellular heterogeneity within many organisms, but few methods have been developed for microbial clonal populations. The yeast genome displays unusually dense transcript ...
Rhodium-Catalyzed Pauson−Khand Reaction Using a Small-Bite-Angle P‑Stereogenic C1‑Diphosphine Ligand
The asymmetric Pauson−Khand reaction catalyzed by [Rh(COD)(MaxPHOS)]BF4 is described. Several 1,6-enynes have been chosen as model substrates affording moderate yields and selectivities of up to 86% ee. Besides binap-type ...
Alterations in the abundance of protamine proteoforms related to sperm chromatin packaging, obesity, and age in normozoospermic men
Protamines are considered among the most relevant sperm proteins because of their functional implications on paternal genome packaging and protection. Although the proteomic evaluation of protamines is technically challenging, ...
Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration
Intestinal organoids capture essential features of the intestinal epithelium such as crypt folding, cellular compartmentalization and collective movements. Each of these processes and their coordination require patterned ...
The tomato genome sequence providies insights into fleshy fruit evolution
Tomato (Solanum lycopersicum) is a major crop plant and a model system for fruit development. Solanum is one of the largest angiosperm genera1 and includes annual and perennial plants from diverse habitats. Here we present ...
Re-analysis of public genetic data reveals a rare X-chromosomal variant associated with type 2 diabetes
The reanalysis of existing GWAS data represents a powerful and cost-effective opportunity to gain insights into the genetics of complex diseases. By reanalyzing publicly available type 2 diabetes (T2D) genome-wide association ...
Higher circulating Vitamin B12 is associated with lower levels of inflammatory markers in individuals at high cardiovascular risk and in naturally aged mice
Vitamin B12 is an essential nutrient that is involved in numerous physiological processes, and its deficiency can lead to various complications, including neurological and haematological disorders. Some studies have suggested ...
The lysosomal proteome of senescent cells contributes to the senescence secretome
Senescent cells accumulate in tissues over time, favoring the onset and progression of multiple age-related diseases. Senescent cells present a remarkable increase in lysosomal mass and elevated autophagic activity. Here, ...
TGFβ drives immune evasion in genetically reconstituted colon cancer metastasis
Most patients with colorectal cancer die as a result of the disease spreading to other organs. However, no prevalent mutations have been associated with metastatic colorectal cancers1,2. Instead, particular features of the ...
Overcoming TGFβ-mediated immune evasion in cancer
This Review discusses the context-dependent functions of transforming growth factor-beta (TGF beta) with regard to the composition and behaviour of different cell populations in the tumour immune microenvironment, as well ...
A new paradigm for molecular dynamics databases: the COVID-19 database, the legacy of a titanic community effort
Molecular dynamics (MD) simulations are keeping computers busy around the world, generating a huge amount of data that is typically not open to the scientific community. Pioneering efforts to ensure the safety and reusability ...
Can we predict and prevent specific sites of metastases in breast cancer patients?
Despite improvements in breast cancer therapies, cancer cells frequently spread to distant organs years or decades after primary tumor surgery and adjuvant treatment. This expansion, known as metastasis, can bring about ...
Formation of left-handed helices by C2′-fluorinated nucleic acids under physiological salt conditions
Recent findings in cell biology have rekindled interest in Z-DNA, the left-handed helical form of DNA. We report here that two minimally modified nucleosides, 2'F-araC and 2'F-riboG, induce the formation of the Z-form under ...
