Institut de Recerca Biomèdica (IRB Barcelona): Enviaments recents
Ara mostrant els elements 1-20 de 418
Expansion of the neocortex and protection from neurodegeneration by in vivo transient reprogramming
Yamanaka factors (YFs) can reverse some aging features in mammalian tissues, but their effects on the brain remain largely unexplored. Here, we induced YFs in the mouse brain in a controlled spatiotemporal manner in two ...
Nuclear stiffness through lamin A/C overexpression differentially modulates chromosomal instability biomarkers
Background Information Mitosis is crucial for the faithful transmission of genetic material, and disruptions can result in chromosomal instability (CIN), a hallmark of cancer. CIN is a known driver of tumor heterogeneity ...
Copy number losses of oncogenes and gains of tumor suppressor genes generate common driver mutations
Cancer driver genes can undergo positive selection for various types of genetic alterations, including gain-of-function or loss-of-function mutations and copy number alterations (CNA). We investigated the landscape of ...
DiffInvex identifies evolutionary shifts in driver gene repertoires during tumorigenesis and chemotherapy
Somatic cells can transform into tumors due to mutations, and the tumors further evolve towards increased aggressiveness and therapy resistance. We develop DiffInvex, a framework for identifying changes in selection acting ...
Rare but specific: 5-bp composite motifs define SMAD binding in BMP signaling
BackgroundReceptor-activated SMADs trimerize with SMAD4 to regulate context-dependent target gene expression. However, the presence of a single SMAD1/5/8 binding motif in cis-regulatory elements alone does not trigger ...
Sex and smoking bias in the selection of somatic mutations in human bladder
Men are at higher risk of several cancer types than women1. For bladder cancer the risk is four times higher for reasons that are not clear2. Smoking is also a principal risk factor for several tumour types, including ...
Flexibility in PAM recognition expands DNA targeting in xCas9
xCas9 is an evolved variant of the CRISPR-Cas9 genome editing system, engineered to improve specificity and reduce undesired off-target effects. How xCas9 expands the DNA targeting capability of Cas9 by recognising a series ...
The pseudotorsional space of RNA
The characterization of the conformational landscape of the RNA backbone is rather complex due to the ability of RNA to assume a large variety of conformations. These backbone conformations can be depicted by pseudotorsional ...
Repression of endogenous retroviruses prevents antiviral immune response and is required for mammary gland development
The role of heterochromatin in cell fate specification during development is unclear. We demonstrate that loss of the lysine 9 of histone H3 (H3K9) methyltransferase G9a in the mammary epithelium results in de novo chromatin ...
Keep calm and transcribe on: chromatin changes with age, but transcription can learn to live with it
Assessing age-related tissue dysfunction represents an emerging field and involves analyses that are far from trivial, often requiring the integration of several largescale (“omic”) techniques. In their recent work, Tessarz ...
Lipids in the tumor microenvironment: immune modulation and metastasis
Tumor cells can undergo metabolic adaptations that support their growth, invasion, and metastasis, such as reprogramming lipid metabolism to meet their energy demands and to promote survival in harsh microenvironmental ...
Common pathophysiology for ANXA11 disorders caused by aspartate 40 variants
Mutations in ANXA11 cause amyotrophic lateral sclerosis (ALS) and have recently been identified as a cause of multisystem proteinopathy and adult-onset muscular dystrophy. These conditions are adult-onset diseases and ...
Transcriptional heterogeneity shapes stress-adaptive responses in yeast
In response to stress, cells activate signaling pathways that coordinate broad changes in gene expression to enhance cell survival. Remarkably, complex variations in gene expression occur even in isogenic populations and ...
G6PD overexpression protects from oxidative stress and age-related hearing loss
Aging of the auditory system is associated with the incremental production of reactive oxygen species (ROS) and the accumulation of oxidative damage in macromolecules, which contributes to cellular malfunction, compromises ...
Enantioselective Ir-Catalyzed Hydrogenation of Terminal Homoallyl Sulfones: Total Synthesis of (-)-Curcumene
A novel methodology for the preparation of chiral methyl benzylic compounds is reported. Terminal homoallyl sulfones were prepared from homoallyl alcohols, which are easily accessible through the recently reported Lewis ...
Dissection of two routes to naïve pluripotency using different kinase inhibitors
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, ...
A genome editing approach to study cancer stem cells in human tumors
The analysis of stem cell hierarchies in human cancers has been hampered by the impossibility of identifying or tracking tumor cell populations in an intact environment. To overcome this limitation, we devised a strategy ...
Efficient siRNA-peptide conjugation for specific targeted delivery into tumor cells
Despite the broad applicability of the Huisgen cycloaddition reaction, the click functionalization of RNAs with peptides remains still a challenge. Here we describe a straightforward method for the click functionalization ...
