Biomedicina: Enviaments recents
Ara mostrant els elements 661-680 de 779
Increased Phospho-AKT in Blood Cells from LRRK2 G2019S Mutation Carriers
The purpose of this study was to investigate whether differential phosphorylation states of blood markers can identify patients with LRRK2 Parkinson's disease (PD). We assessed phospho(P)-Ser-935-LRRK2 and P-Ser-473-AKT ...
Cell Banking of HEK293T cell line for clinical-grade lentiviral particles manufacturing
Background: Cell banks are widely used to preserve cell properties as well as to record and control the use of cell lines in biomedical research. The generation of cell banks for the manufacturing of Advanced Therapy ...
Increased levels of rictor prevent mutant huntingtin-induced neuronal degeneration
Rictor associates with mTOR to form the mTORC2 complex, which activity regulates neuronal function and survival. Neurodegenerative diseases are characterized by the presence of neuronal dysfunction and cell death in specific ...
Preschool teachers display a flexible pattern of pedagogical actions in promoting healthy habits in children
The school represents the optimal setting for promoting the physical, emotional, and social health of children, especially during the first years of life. Understanding the pedagogical actions of teachers to address health ...
Synaptic RTP801 contributes to motor-learning dysfunction in Huntington's disease
RTP801/REDD1 is a stress-responsive protein that mediates mutant huntingtin (mhtt) toxicity in cellular models and is up regulated in Huntington's disease (HD) patients' putamen. Here, we investigated whether RTP801 is ...
Pyk2 Regulates MAMs and Mitochondrial Dynamics in Hippocampal Neurons
Pyk2 is a non-receptor tyrosine kinase enriched in hippocampal neurons, which can be activated by calcium-dependent mechanisms. In neurons, Pyk2 is mostly localised in the cytosol and dendritic shafts but can translocate ...
TRESK background K+ channel deletion selectively uncovers enhanced mechanical and cold sensitivity
Background potassium-permeable ion channels play a critical role in tuning the excitability of nociceptors, yet the precise role played by different subsets of channels is not fully understood. Decreases in TRESK (TWIK-related ...
Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Protects Striatal Cells and Improves Motor Function in Huntington's Disease Models: Role of PAC1 Receptor.
Huntington's disease (HD) is a hereditary neurodegenerative disorder caused by the expression of mutant huntingtin (mHtt). One of the main features of HD is the degeneration of the striatum that leads to motor discoordination. ...
The background K+ channel TRESK in sensory physiology and pain
TRESK belongs to the K2P family of potassium channels, also known as background or leak potassium channels due to their biophysical properties and their role regulating membrane potential of cells. Several studies to date ...
In utero and childhood exposure to tobacco smoke and multi-layer molecular signatures in children
Antecedents: S'han informat àmpliament dels efectes adversos sobre la salut de l'exposició primerenca al tabac. Malgrat això, els mecanismes moleculars subjacents de l'exposició in utero i postnatal al fum del tabac només ...
GPC3-Unc5 receptor complex structure and role in cell migration
Neural migration is a critical step during brain development that requires the interactions of cell-surface guidance receptors. Cancer cells often hijack these mechanisms to disseminate. Here, we reveal crystal structures ...
Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs
Human pluripotent stem cells (hPSCs) have generated unprecedented interest in the scientific community, given their potential applications in regenerative medicine, disease modeling, toxicology and drug screening. However, ...
Age-related changes in STriatal-Enriched protein tyrosine Phosphatase levels: Regulation by BDNF
Recent results indicate that STriatal-Enriched protein tyrosine Phosphatase (STEP) levels are regulated by brain-derived neurotrophic factor (BDNF), whose expression changes during postnatal development and aging. Here, ...
Placental transfer of NMDAR antibodies causes reversible alterations in mice
Objective: To determine whether maternofetal transfer of NMDA receptor (NMDAR) antibodies has pathogenic effects on the fetus and offspring, we developed a model of placental transfer of antibodies. Methods: Pregnant ...
Tbr1 Misexpression Alters Neuronal Development in the Cerebral Cortex
Changes in the transcription factor (TF) expression are critical for brain development, and they may also underlie neurodevelopmental disorders. Indeed, T-box brain1 (Tbr1) is a TF crucial for the formation of neocortical ...
Isoform-Specific Reduction of the Basic Helix-Loop-Helix Transcription Factor TCF4 Levels in Huntington's Disease
Huntington's disease (HD) is an inherited neurodegenerative disorder with onset of characteristic motor symptoms at midlife, preceded by subtle cognitive and behavioral disturbances. Transcriptional dysregulation emerges ...
Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration
Intestinal organoids capture essential features of the intestinal epithelium such as crypt folding, cellular compartmentalization and collective movements. Each of these processes and their coordination require patterned ...
Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium
Epithelial repair and regeneration are driven by collective cell migration and division. Both cellular functions involve tightly controlled mechanical events, but how physical forces regulate cell division in migrating ...
Aberrant epigenome in iPSC-derived dopaminergic neurons from Parkinson's disease patients
The epigenomic landscape of Parkinson's disease (PD) remains unknown. We performed a genomewide DNA methylation and a transcriptome studies in induced pluripotent stem cell (iPSC)-derived dopaminergic neurons (DAn) generated ...
A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea
Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a critical role in the pathogenesis of OSA-associated morbidities, especially in the cardiovascular and respiratory systems. Oxidative stress ...
