Articles publicats Departament de Biologia: Recent submissions
Now showing items 21-40 of 408
How do lifestyle and environmental factors influence the sperm epigenome? Effects on sperm fertilising ability, embryo development and offspring health
Recent studies support the influence of paternal lifestyle and diet before conception on the health of the offspring via epigenetic inheritance through sperm DNA methylation, histone modification, and small non-coding RNA ...
Evaluation of sperm quality and male fertility: The use of molecular markers in boar sperm and seminal plasma
In pig production, the optimization of artificial insemination (AI) efficiency significantly relies on the accurate assessment of semen quality and fertility of boars. Traditional methods such as conventional seminogram ...
Deep brain stimulation of the medial forebrain bundle promotes the extinction of active avoidance and is associated with mossy fibber sprouting in the hippocampus
Background: Post-traumatic stress disorder (PTSD) causes intrusive symptoms and avoidance behaviours due to dysregulation in various brain regions, including the hippocampus. Deep brain stimulation (DBS) shows promise for ...
Long-term storage of boar seminal doses contaminated with Proteus vulgaris: a dose-dependent effect on sperm motility and sperm-bacteria interaction
This study evaluated how Proteus vulgaris affects sperm quality and sperm-bacteria interaction in stored semen samples. A strain of P. vulgaris resistant to streptomycin, penicillin, lincomycin and spectinomycin was added ...
DNA barcoding reveals widespread co-occurrence of other chub species in the range of the endemic Catalan chub, Squalius laietanus
The Catalan chub, Squalius laietanus, was described in 2007 and has recently reached Vulnerable status on the IUCN Red List of Threatened Species. The assigned range for this species includes the lower course of the Ebro ...
Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels
The main aim of the present study was to determine whether differences in the amounts of free cysteine residues in sperm nucleoproteins, which are a direct marker of the integrity of the disulfide bonds between nucleoproteins, ...
Clustering of spermatozoa examined through flow cytometry provides more information than the conventional assessment: a resilience to osmotic stress example
Context: Conventional sperm quality tests may not be sufficient to predict the fertilising ability of a given ejaculate; thus, rapid, reliable and sensitive tests are necessary to measure sperm function. Aims: This study ...
Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
Prostaglandin F2α (PGF2α) has been used to improve reproductive performance in swine. The goal of the present work was to determine how the addition of PGF2α affects boar sperm quality. Eleven different treatments were ...
Predictive indicators of cryotolerance and fertility in bovine sperm: evaluating fresh semen quality to improve AI outcomes with frozen-thawed sperm
The success of artificial insemination (AI) with frozen-thawed semen in cattle is influenced by both female factors and sperm quality. In terms of sperm quality, prior studies indicate that the ability of frozen-thawed ...
Hyaluronic acid delays boar sperm capacitation after 3 days of storage at 15 °C
The present study was undertaken to determine the effects of the addition of hyaluronic acid (HA), ranged from 12.5 to 200 μg/ml, on boar sperm capacitation status during a storage time (up to 3 days) at 15 °C in Beltsville ...
SARS-CoV-2 in river water: Implications in low sanitation countries
Since the beginning of COVID-19 pandemic studies on viral shedding have reported that this virus is excreted in feces in most patients. High viral loads are found at the sewage pipeline or at the entrance of wastewater ...
Silibinin is a suppressor of the metastasis-promoting transcription factor ID3
Background: ID3 (inhibitor of DNA binding/differentiation-3) is a transcription factor that enables metastasis by promoting stem cell-like properties in endothelial and tumor cells. The milk thistle flavonolignan silibinin ...
Escherichia coli as a New Platform for the Fast Production of Vault-like Nanoparticles: An Optimized Protocol
Vaults are protein nanoparticles that are found in almost all eukaryotic cells but are absent in prokaryotic ones. Due to their properties (nanometric size, biodegradability, biocompatibility, and lack of immunogenicity), ...
Genomic Analysis of the Giant Red Shrimp (Aristaeomorpha foliacea) Using Next-Generation Sequencing: Set of Tools for Population Studies
The giant red shrimp, Aristaeomorpha foliacea, is a valuable marine fishing resource. The conservation of species, especially exploited ones, depends on a good knowledge of their biology, as well as the development of ...
Sperm methylome profiling can discern fertility levels in the porcine biomedical model
A combined Genotyping By Sequencing (GBS) and methylated DNA immunoprecipita-tion (MeDIP) protocol was used to identify—in parallel—genetic variation (Genomic-Wide Association Studies (GWAS) and epigenetic differences of ...
Protocol to assess rewarding brain stimulation as a learning and memory modulating treatment: Comparison between self-administration and experimenter-administration
Intracranial electrical self-stimulation (ICSS) is a useful procedure in animal research. This form of administration ensures that areas of the brain reward system (BRS) are being functionally activated, since the animals ...
An Algorithm to Predict the Lack of Pregnancy after Intrauterine Insemination in Infertile Patients
Increasing intrauterine insemination (IUI) success rates is essential to improve the quality of care for infertile couples. Additionally, straight referral of couples with less probability of achieving a pregnancy through ...
Editorial: Resistant pathogens: from distribution to emerging therapies
Editorial on the research topic resistant pathogens, from distribution to emerging therapies
Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers
The extensive use of antibiotics in human and veterinary medicine and their subsequent release into the environment may have direct consequences for autochthonous bacterial communities, especially in freshwater ecosystems. ...
ppGpp, the General Stress Response Alarmone, Is Required for the Expression of the α-Hemolysin Toxin in the Uropathogenic Escherichia coli Isolate, J96
ppGpp is an intracellular sensor that, in response to different types of stress, coordinates the rearrangement of the gene expression pattern of bacteria to promote adaptation and survival to new environmental conditions. ...
