Articles publicats Departament de Biologia: Envíos recientes
Mostrando ítems 41-60 de 408
Ligation of multiple protein domains using orthogonal inteins with non-native splice junctions
Protein splicing is a self-catalyzed process in which an internal protein domain (the intein) is excised from its flanking sequences, linking them together with a canonical peptide bond. Trans-inteins are separated in two ...
Insights into crucial molecules and protein channels involved in pig sperm cryopreservation
Cryopreservation is the most efficient procedure for long-term preservation of mammalian sperm; however, its use is not currently dominant for boar sperm before its use for artificial insemination. In fact, freezing and ...
Characterization of Latin American migrants at risk for Trypanosoma cruzi infection in a non-endemic setting. Insights into initial evaluation of cardiac and digestive involvement
Trypanosoma cruzi causes Chagas disease (CD), a potentially fatal disease characterized by cardiac disorders and digestive, neurological or mixed alterations. T. cruzi is transmitted to humans by the bite of triatomine ...
Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatin
Background: Sperm cryopreservation is widely used in the cattle industry, as it allows for disassociating the localization of sires and the collection of semen from the timing of artificial insemination. While freeze-thawing ...
Assessment of Alinity s Chagas® as a Primary Diagnostic Test for Chronic Chagas Disease in a Non-Endemic Area of Europe (Barcelona, Spain)
Chagas disease (CD) has become a worldwide problem due to globalization. In Europe, most cases are imported and are diagnosed in the chronic phase by two serological tests, as recommended by the World Health Organization. ...
Occurrence and persistence of carbapenemases genes in hospital and wastewater treatment plants and propagation in the receiving river
This study aims to investigate the prevalence of clinically relevant carbapenemases genes (blaKPC, blaNDM and blaOXA-48) in water samples collected over one-year period from hospital (H), raw and treated wastewater of two ...
Sperm chromatin: evaluation, epigenetic signatures and relevance for embryo development and assisted reproductive technology outcomes
Sperm chromatin is distinct from somatic cell chromatin, as a result of extensive remodeling during the final stages of spermatogenesis. In this process, the majority of histones is replaced with protamines. The chromatin ...
Identification of differences in gene expression implicated in the Adherent-Invasive Escherichia coli phenotype during in vitro infection of intestinal epithelial cells
Introduction: Adherent-invasive Escherichia coli (AIEC) is strongly associated with the pathogenesis of Crohn’s disease (CD). However, no molecular markers currently exist for AIEC identification. This study aimed to ...
Antibiotic resistance in urban and hospital wastewaters and their impact on a receiving freshwater ecosystem
The main objective of this study was to investigate the antibiotic resistance (AR) levels in wastewater (WW) and the impact on the receiving river. Samples were collected once per season over one year in the WW of a hospital, ...
Worldwide Control and Management of Chagas Disease in a New Era of Globalization: a Close Look at Congenital Trypanosoma cruzi Infection
Population movements have turned Chagas disease (CD) into a global public health problem. Despite the successful implementation of subregional initiatives to control vectorial and transfusional Trypanosoma cruzi transmission ...
Evidence of Alternative Splicing as a Regulatory Mechanism for Kissr2 in Pejerrey Fish
Kisspeptin receptors are G-Protein-Coupled Receptors that regulate GnRH synthesis and release in vertebrates. Here, we report the gene structure of two kisspeptin receptors (kissr2 and kissr3) in pejerrey fish. Genomic ...
Reagent-free phosphorus precipitation from a denitrified swine effluent in a batch electrochemical system
There is high interest in the recovery of phosphorus (P) from wastewater through crystallization processes. However, the addition of chemical reagents (e.g., sodium hydroxide) to raise the pH may result in high treatment ...
Antibiotic resistance along an urban river impacted by treated wastewaters
Urban rivers are impacted ecosystems which may play an important role as reservoirs for antibiotic-resistant (AR) bacteria. The main objective of this study was to describe the prevalence of antibiotic resistance along a ...
Heat Shock Protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 ºC
At present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing for the maintenance of cell lifespan. In this context, the study of proteins ...
Real‐time genomics for One Health
The ongoing degradation of natural systems and other environmental changes has put our society at a crossroad with respect to our future relationship with our planet. While the concept of One Health describes how human ...
Seminal Extracellular Vesicles and Their Involvement in Male (In)Fertility: A Systematic Review
Seminal plasma contains numerous extracellular vesicles (sEVs). Since sEVs are apparently involved in male (in)fertility, this systematic review focused on studies specifically investigating such relationship. Embase, ...
Contribution of Toxin–Antitoxin Systems to Adherent-Invasive E. coli Pathogenesis
Pathobionts have been implicated in various chronic diseases, including Crohn’s disease (CD), a multifactorial chronic inflammatory condition that primarily affects the gastrointestinal tract, causing inflammation and ...
Pro-Oxidant Activity of Amine-Pyridine-Based Iron Complexes Efficiently Kills Cancer and Cancer Stem-Like Cells
Differential redox homeostasis in normal and malignant cells suggests that pro-oxidantinduced upregulation of cellular reactive oxygen species (ROS) should selectively target cancer cells without compromising the viability ...
A Nuclear-Directed Ribonuclease Variant Targets Cancer Stem Cells and Inhibits Migration and Invasion of Breast Cancer Cells
Despite the significant advances in cancer research made in recent years, this disease remains one of the leading causes of death worldwide. In part, this is due to the fact that after therapy, a subpopulation of self-renewing ...
Surveillance of SARS-CoV-2 in sewage from buildings housing residents with different vulnerability levels
During the last three years, various restrictions have been set up to limit the transmission of the Coronavirus Disease (COVID-19). While these rules apply at a large scale (e.g., country-wide level) human-to-human ...
