Título:
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Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices
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Autor/a:
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Wolfenson, Haguy; Meacci, Giovanni; Liu, Shuaimin; Stachowiak, Matthew R.; Iskratsch, Thomas; Ghassemi, Saba; Roca-Cusachs Soulere, Pere; Shaughnessy, Ben O'; Hone, James; Sheetz, Michael P.
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Otros autores:
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Universitat de Barcelona |
Abstract:
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Cells test the rigidity of the extracellular matrix by applying forces to it through integrin adhesions. Recent measurements show that these forces are applied by local micrometre-scale contractions, but how contraction force is regulated by rigidity is unknown. Here we performed high temporal- and spatial-resolution tracking of contractile forces by plating cells on sub-micrometre elastomeric pillars. We found that actomyosin-based sarcomere-like contractile units (CUs) simultaneously moved opposing pillars in net steps of ∼2.5 nm, independent of rigidity. What correlated with rigidity was the number of steps taken to reach a force level that activated recruitment of α-actinin to the CUs. When we removed actomyosin restriction by depleting tropomyosin 2.1, we observed larger steps and higher forces that resulted in aberrant rigidity sensing and growth of non-transformed cells on soft matrices. Thus, we conclude that tropomyosin 2.1 acts as a suppressor of growth on soft matrices by supporting proper rigidity sensing. |
Materia(s):
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-Matriu extracel·lular -Extracellular matrix |
Derechos:
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(c) Wolfenson, Haguy et al., 2016
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Tipo de documento:
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Artículo Artículo - Versión aceptada |
Editor:
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Nature Publishing Group
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