Late Jurassic and Early Cretaceous charophyte assemblages from the northern part of the Aquitaine Basin in south-west France are reviewed here to understand their palaeoecological, palaeobiogeographical and biostratigraphic features. Three sites were studied: the Tithonian-lower Berriasian of Chassiron, and the Berriasian of Cherves-de-Cognac and Angeac-Charente. Abundant porocharaceans, less abundant clavatoraceans and scarce characeans recorded in Cherves-de-Cognac and Angeac-Charente indicate that brackish water environments were substituted by freshwater environments eastwards. The occurrence of Clavator grovesii var. grovesii and morphotypes intermediate with C. grovesii var. discordis in the same areas is significant from a biostratigraphic viewpoint, since these species belong to the Maillardii, Incrassatus and Nurrensis European charophyte biozones, representing the Berriasian. This observation refutes a previous dating of the Angeac-Charente site and highlights the absence of Hauterivian-Barremian records in northern Aquitaine, which is in contrast to the more complete Lower Cretaceous record in southern Aquitaine. These contrasting records could be due to differences in the available sedimentary space produced by the opening of the Bay of Biscay during the Barremian.