Title:
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Numerical simulation of orbitally shaken viscous fluids with free surface
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Author:
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Discacciati, Marco; Hacker, David; Quarteroni, A.; Quinodoz, Samuel; Tissot, Stéphanie; Wurm, M. Florian
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Other authors:
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Universitat Politècnica de Catalunya. LACÀN - Mètodes Numèrics en Ciències Aplicades i Enginyeria |
Abstract:
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Orbitally shaken bioreactors are an emerging alternative to stirred-tank bioreactors for large-scale mam- malian cell culture, but their fluid dynamics is still not well defined. Among the theoretical and practical issues that remain to be resolved, the characterization of the liquid free surface during orbital shaking remains a major challenge because it is an essential aspect of gas transfer and mixing in these reactors. To simulate the fluid behavior and the free surface shape, we developed a numerical method based on the finite element framework. We found that the large density ratio between the liquid and the gas phases induced unphysical results for the free surface shape. We therefore devised a new pressure correction scheme to deal with large density ratios. The simulations operated with this new scheme gave values of wave amplitude similar to the ones mea- sured experimentally. These simulations were used to calculate the shear stress and to study the mixing principle in orbitally shaken bioreactors |
Abstract:
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Peer Reviewed |
Subject(s):
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-Àrees temàtiques de la UPC::Matemàtiques i estadística::Anàlisi numèrica::Mètodes en elements finits -Numerical analysis -Finite elements -Free surface -Level set -Multi-phase flows -Navier-Stokes -Anàlisi numèrica -Classificació AMS::74 Mechanics of deformable solids::74S Numerical methods |
Rights:
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http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Document type:
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Article - Submitted version Article |
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