CERKL, a retinal disease gene,encodes an mRNA-biding protein that localizes in compact and unstranslated mRNPs associated to microtubules.

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RECERCAT Home > Universitat de Barcelona > Genètica, Microbiologia i Estadística > Articles publicats en revistes (Genètica, Microbiologia i Estadística) > View document

To access the full text documents, please follow this link: http://hdl.handle.net/2445/124658

dc.contributor	Universitat de Barcelona
dc.contributor.author	Fathinajafabadi, Alihamze
dc.contributor.author	Pérez-Jiménez, Eva
dc.contributor.author	Riera, Marina
dc.contributor.author	Knecht, Erwin
dc.contributor.author	Gonzàlez-Duarte, Roser
dc.date	2018-09-18T11:58:55Z
dc.date	2018-09-18T11:58:55Z
dc.date	2014-02-03
dc.date	2018-09-18T11:58:55Z
dc.identifier.citation	1932-6203
dc.identifier.citation	632970
dc.identifier.uri	http://hdl.handle.net/2445/124658
dc.description.abstract	The function of CERKL (CERamide Kinase Like), a causative gene of retinitis pigmentosa and cone-rod dystrophy, still awaits characterization. To approach its cellular role we have investigated the subcellular localization and interaction partners of the full length CERKL isoform, CERKLa of 532 amino acids, in different cell lines, including a photoreceptor-derived cell line. We demonstrate that CERKLa is a main component of compact and untranslated mRNPs and that associates with other RNP complexes such as stress granules, P-bodies and polysomes. CERKLa is a protein that binds through its N-terminus to mRNAs and interacts with other mRNA-binding proteins like eIF3B, PABP, HSP70 and RPS3. Except for eIF3B, these interactions depend on the integrity of mRNAs but not of ribosomes. Interestingly, the C125W CERKLa pathological mutant does not interact with eIF3B and is absent from these complexes. Compact mRNPs containing CERKLa also associate with microtubules and are found in neurites of neural differentiated cells. These localizations had not been reported previously for any member of the retinal disorders gene family and should be considered when investigating the pathogenic mechanisms and therapeutical approaches in these diseases.
dc.format	13 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Public Library of Science (PLoS)
dc.relation	Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0087898
dc.relation	PLoS One, 2014, vol. 9, num. 2, p. 1-13
dc.relation	https://doi.org/10.1371/journal.pone.0087898
dc.rights	cc-by (c) Fathinajafabadi, Alihamze et al., 2014
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by/3.0/es
dc.subject	Ribonucleases
dc.subject	Citoplasma
dc.subject	Microtúbuls
dc.subject	Malalties de la retina
dc.subject	Ribonucleases
dc.subject	Cytoplasm
dc.subject	Microtubules
dc.subject	Retinal diseases
dc.title	CERKL, a retinal disease gene,encodes an mRNA-biding protein that localizes in compact and unstranslated mRNPs associated to microtubules.
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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