dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Lizana, X. |
dc.contributor.author |
López Sevilla, Asunción |
dc.contributor.author |
Benito, S. |
dc.contributor.author |
Agustí, Gemma |
dc.contributor.author |
Ríos Alcolea, Martín |
dc.contributor.author |
Piqué i Clusella, Núria |
dc.contributor.author |
Marqués Villavecchia, Ana M. |
dc.contributor.author |
Codony, F. |
dc.date |
2018-04-18T13:09:01Z |
dc.date |
2018-12-31T06:10:22Z |
dc.date |
2017-11 |
dc.date |
2018-04-18T13:09:02Z |
dc.identifier.citation |
1438-4639 |
dc.identifier.citation |
675570 |
dc.identifier.uri |
http://hdl.handle.net/2445/121682 |
dc.format |
7 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Elsevier |
dc.relation |
Versió postprint del document publicat a: https://doi.org/10.1016/j.ijheh.2017.08.007 |
dc.relation |
International Journal of Hygiene and Environmental Health, 2017, vol. 220, num. 8, p. 1318-1324 |
dc.relation |
https://doi.org/10.1016/j.ijheh.2017.08.007 |
dc.rights |
cc-by-nc-nd (c) Urban and Fischer, 2017 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/es |
dc.subject |
Legionel·la |
dc.subject |
Reacció en cadena de la polimerasa |
dc.subject |
ADN |
dc.subject |
Bacteris |
dc.subject |
Anàlisi de l'aigua |
dc.subject |
Legionella |
dc.subject |
Polymerase chain reaction |
dc.subject |
DNA |
dc.subject |
Bacteria |
dc.subject |
Water analysis |
dc.title |
Viability qPCR, a new tool for Legionella risk management |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/acceptedVersion |
dc.description.abstract |
Background Viability quantitative Polymerase Chain Reaction (v-qPCR) is a recent analytical approach for only detecting live microorganisms by DNA amplification-based methods This approach is based on the use of a reagent that irreversibly fixes dead cells DNA. In this study, we evaluate the utility of v-qPCR versus culture method for Legionellosis risk management. Methods The present study was performed using 116 real samples. Water samples were simultaneously analysed by culture, v-qPCR and qPCR methods. Results were compared by means of a non-parametric test. Results In 11.6% of samples using both methods (culture method and v-qPCR) results were positive, in 50.0% of samples both methods gave rise to negative results. As expected, equivalence between methods was not observed in all cases, as in 32.1% of samples positive results were obtained by v-qPCR and all of them gave rise to negative results by culture. Only in 6.3% of samples, with very low Legionella levels, was culture positive and v-qPCR negative. In 3.5% of samples, overgrowth of other bacteria did not allow performing the culture. When comparing both methods, significant differences between culture and v-qPCR were in the samples belonging to the cooling towers-evaporative condensers group. The v-qPCR method detected greater presence and obtained higher concentrations of Legionella spp. (p < 0.001). Otherwise, no significant differences between methods were found in the rest of the groups. Conclusions The v-qPCR method can be used as a quick tool to evaluate Legionellosis risk, especially in cooling towers-evaporative condensers, where this technique can detect higher levels than culture. The combined interpretation of PCR results along with the ratio of live cells is proposed as a tool for understanding the sample context and estimating the Legionellosis risk potential according to 4 levels of hierarchy. |