dc.contributor.author |
Madrid, Lola |
dc.contributor.author |
Varo, Rosauro |
dc.contributor.author |
Maculuve, Sónia Amós |
dc.contributor.author |
Nhampossa, Tacilta |
dc.contributor.author |
Muñoz-Almagro, Carmen |
dc.contributor.author |
Calderon, Enrique J. |
dc.contributor.author |
Esteva, Cristina |
dc.contributor.author |
Carrilho, Carla |
dc.contributor.author |
Ismail, Mamudo R. |
dc.contributor.author |
Vieites, Begoña |
dc.contributor.author |
Friaza, Vicente |
dc.contributor.author |
Lozano Dominguez, María del Carmen |
dc.contributor.author |
Menéndez, Clara |
dc.contributor.author |
Bassat Orellana, Quique |
dc.date |
2018-03-23T13:00:43Z |
dc.date |
2018-03-23T13:00:43Z |
dc.date |
2018-03-14 |
dc.date |
2018-03-21T18:59:48Z |
dc.identifier.citation |
1932-6203 |
dc.identifier.uri |
http://hdl.handle.net/2445/121075 |
dc.format |
17 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Public Library of Science (PLoS) |
dc.relation |
Reproducció del document publicat a:
http://dx.doi.org/10.1371/journal.pone.0194186 |
dc.relation |
PLoS One, 2018, vol. 13, num. 3, p. e0194186 |
dc.relation |
http://dx.doi.org/10.1371/journal.pone.0194186 |
dc.rights |
cc by (c) Madrid et al., 2018 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es/ |
dc.subject |
Infants nadons |
dc.subject |
Citomegalovirus |
dc.subject |
Newborn infants |
dc.subject |
Cytomegaloviruses |
dc.title |
Congenital cytomegalovirus, parvovirus and enterovirus infection
in Mozambican newborns at birth: A cross-sectional survey |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
BACKGROUND: Congenital cytomegalovirus (cCMV) infection is the
most prevalent congenital infection acquired worldwide, with
higher incidence in developing countries and among HIV-exposed
children. Less is known regarding vertical transmission of
parvovirus B19 (B19V) and enterovirus (EV). We aimed to assess
the prevalence of CMV, B19V and EV vertical transmission and
compare results of screening of congenital CMV obtained from two
different specimens in a semirural Mozambican maternity.
METHODS: A cross sectional study was conducted among pregnant
mothers attending Manhica District Hospital upon delivery.
Information on maternal risk factors was ascertained. Dried
umbilical cord (DUC) samples were collected in filter paper for
CMV, B19V and EV detection by real-time polymerase chain
reaction (RT-PCR), and nasopharyngeal aspirates (NPA) to test
for CMV by RT-PCR. Maternal blood samples and placental biopsy
samples were also obtained to investigate CMV maternal serology,
HIV status and immunopathology. RESULTS: From September 2014 to
January 2015, 118 mothers/newborn pairs were recruited.
Prevalence of maternal HIV infection was 31.4% (37/118). CMV
RT-PCR was positive in 3/115 (2.6%) of DUC samples and in 3/96
(6.3%) of NPA samples obtained from neonates. The concordance of
the RT-PCR assay through DUC with their correspondent NPA sample
was moderate (Kappa = 0.42 and p<0.001. No differences on
cCMV prevalence were found among HIV-exposed and unexposed. All
(100%) mothers were seropositive for CMV IgG. RT-PCR of EV and
B19V in DUC were both negative in all screened cases. No
histological specific findings were found in placental tissues.
No risk factors associated to vertical transmission of these
viral infections were found. CONCLUSIONS: This study indicates
the significant occurrence of vertical transmission of CMV in
southern Mozambique. Larger studies are needed to evaluate the
true burden, clinical relevance and consequences of congenital
infections with such pathogens in resource-constrained settings. |