Title:
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The incC sequence is required for R27 plasmid stability
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Author:
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Tassinari, Eleonora; Aznar Vicente, Sonia; Urcola, Imanol; Prieto, Alejandro; Hüttener Queiroz, Mário; Juárez Giménez, Antonio
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Abstract:
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IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance. |
Subject(s):
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-Seqüència d'aminoàcids -Plasmidis -Amino acid sequence -Plasmids |
Rights:
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cc-by (c) Tassinari, Eleonora et al., 2016
http://creativecommons.org/licenses/by/3.0/es |
Document type:
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Article Article - Published version |
Published by:
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Frontiers Media
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