dc.contributor.author |
Jiménez, Alfons |
dc.contributor.author |
Rees-Channer, Roxanne R. |
dc.contributor.author |
Perera, Rushini |
dc.contributor.author |
Gamboa, Dionicia |
dc.contributor.author |
Chiodini, Peter L. |
dc.contributor.author |
Gonzalez, Iveth J. |
dc.contributor.author |
Mayor Aparicio, Alfredo Gabriel |
dc.contributor.author |
Ding, Xavier C. |
dc.date |
2017-04-11T07:23:17Z |
dc.date |
2017-04-11T07:23:17Z |
dc.date |
2017-03-24 |
dc.date |
2017-04-05T18:01:24Z |
dc.identifier.citation |
1475-2875 |
dc.identifier.uri |
http://hdl.handle.net/2445/109604 |
dc.format |
9 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
BioMed Central |
dc.relation |
Reproducció del document publicat a:
http://dx.doi.org/10.1186/s12936-017-1780-5 |
dc.relation |
Malaria Journal, 2017, vol. 16, num. 128 |
dc.relation |
http://dx.doi.org/10.1186/s12936-017-1780-5 |
dc.rights |
cc by (c) Jiménez et al., 2017 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es/ |
dc.subject |
Malària |
dc.subject |
Diagnòstic |
dc.subject |
Malaria |
dc.subject |
Diagnosis |
dc.title |
Analytical sensitivity of current best-in-class malaria rapid
diagnostic tests |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
BACKGROUND: Rapid diagnostic tests (RDTs) are today the most
widely used method for malaria diagnosis and are recommended,
alongside microscopy, for the confirmation of suspected cases
before the administration of anti-malarial treatment. The
diagnostic performance of RDTs, as compared to microscopy or PCR
is well described but the actual analytical sensitivity of
current best-in-class tests is poorly documented. This value is
however a key performance indicator and a benchmark value needed
to developed new RDTs of improved sensitivity. METHODS: Thirteen
RDTs detecting either the Plasmodium falciparum histidine rich
protein 2 (HRP2) or the plasmodial lactate dehydrogenase (pLDH)
antigens were selected from the best performing RDTs according
to the WHO-FIND product testing programme. The analytical
sensitivity of these products was evaluated using a range of
reference materials including P. falciparum and Plasmodium vivax
whole parasite samples as well as recombinant proteins. RESULTS:
The best performing HRP2-based RDTs could detect all P.
falciparum cultured samples at concentrations as low as 0.8
ng/mL of HRP2. The limit of detection of the best performing
pLDH-based RDT specifically detecting P. vivax was 25 ng/mL of
pLDH. CONCLUSION: The analytical sensitivity of P. vivax and Pan
pLDH-based RDTs appears to vary considerably from product to
product, and improvement of the limit-of-detection for P. vivax
detecting RDTs is needed to match the performance of HRP2 and Pf
pLDH-based RDTs for P. falciparum. Different assays using
different reference materials produce different values for
antigen concentration in a given specimen, highlighting the need
to establish universal reference assays. |