Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

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RECERCAT Principal > Universitat de Barcelona > Bioquímica i Fisiologia > Articles publicats en revistes (Bioquímica i Fisiologia) > Visualizar documento

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dc.contributor	Universitat de Barcelona
dc.contributor.author	Scheler, Ulschan
dc.contributor.author	Brandt, Wolfgang
dc.contributor.author	Porzel, Andrea
dc.contributor.author	Rothe, Kathleen
dc.contributor.author	Manzano Alías, David
dc.contributor.author	Bozic, Dragana
dc.contributor.author	Papaefthimiou, Dimitra
dc.contributor.author	Balcke, Gerd Ulrich
dc.contributor.author	Henning, Anja
dc.contributor.author	Lohse, Swanhild
dc.contributor.author	Marillonnet, Sylvestre
dc.contributor.author	Kanellis, Angelos K.
dc.contributor.author	Ferrer i Prats, Albert
dc.contributor.author	Tissier, Alain
dc.date	2016-11-30T17:35:42Z
dc.date	2016-11-30T17:35:42Z
dc.date	2016-10-05
dc.date	2016-11-30T17:35:47Z
dc.identifier.citation	2041-1723
dc.identifier.citation	664512
dc.identifier.uri	http://hdl.handle.net/2445/104313
dc.format	11 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Nature Publishing Group
dc.relation	Reproducció del document publicat a: https://doi.org/10.1038/ncomms12942
dc.relation	Nature Communications, 2016, vol. 7, p. 1-11
dc.relation	https://doi.org/10.1038/ncomms12942
dc.relation	info:eu-repo/grantAgreement/EC/FP7/227448/EU//TERPMED
dc.rights	cc-by (c) Scheler, Ulschan et al., 2016
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by/3.0/es
dc.subject	Additius alimentaris
dc.subject	Llevats (Botànica)
dc.subject	Biosíntesi
dc.subject	Food additives
dc.subject	Yeast fungi
dc.subject	Biosynthesis
dc.title	Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.description.abstract	Rosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks to their high anti-oxidant activity. We describe here the elucidation of the complete biosynthetic pathway of carnosic acid and its reconstitution in yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis and Salvia fruticosa already characterized as ferruginol synthases are also able to produce 11-hydroxyferruginol. Modelling-based mutagenesis of three amino acids in the related ferruginol synthase (CYP76AH1) from S. miltiorrhiza is sufficient to convert it to a 11-hydroxyferruginol synthase (HFS). The three sequential C20 oxidations for the conversion of 11-hydroxyferruginol to carnosic acid are catalysed by the related CYP76AK6-8. The availability of the genes for the biosynthesis of carnosic acid opens opportunities for the metabolic engineering of phenolic diterpenes, a class of compounds with potent anti-oxidant, anti-inflammatory and anti-tumour activities.

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