A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus

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dc.contributor	Universitat de Barcelona
dc.contributor.author	Blasco, Lucía
dc.contributor.author	Veiga Crespo, Patricia
dc.contributor.author	Viñas, Miquel
dc.contributor.author	Villa, Tomás G.
dc.date	2014-03-21T13:18:31Z
dc.date	2014-03-21T13:18:31Z
dc.date	2011-12-01
dc.date	2014-03-21T13:18:31Z
dc.identifier.citation	1139-6709
dc.identifier.citation	614979
dc.identifier.uri	http://hdl.handle.net/2445/52756
dc.format	6 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Spanish Society for Microbiology (SEM) and Viguera Editores SL
dc.relation	Reproducció del document publicat a: http://dx.doi.org/10.2436/20.1501.01.149
dc.relation	International Microbiology, 2011, vol. 14, p. 201-206
dc.relation	http://dx.doi.org/10.2436/20.1501.01.149
dc.rights	cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2011
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by-nc-sa/3.0/es
dc.subject	Fermentació
dc.subject	Saccharomyces cerevisiae
dc.subject	Genòmica
dc.subject	Vi
dc.subject	Fermentation
dc.subject	Saccharomyces cerevisiae
dc.subject	Genomics
dc.subject	Wine
dc.title	A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.description.abstract	Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species.

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