To access the full text documents, please follow this link: http://hdl.handle.net/2445/36438

Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single cell tracking and traction force microscopy
Nocentini, Sara; Reginensi, Diego; Garcia, Simon; Carulla Martí, Patricia; Moreno-Flores, María Teresa; Wandosell, Francisco; Trepat Guixer, Xavier; Bribián Arruego, Ana; Río Fernández, José Antonio del
Universitat de Barcelona
Newly generated olfactory receptor axons grow from the peripheral to the central nervous system aided by olfactory ensheathing cells (OECs). Thus, OEC transplantation has emerged as a promising therapy for spinal cord injuries and for other neural diseases. However, these cells do not present a uniform population, but, instead, a functionally heterogeneous population that exhibits a variety of responses including adhesion, repulsion and crossover during cell-cell and cell-matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. Here, we demonstrated that rodent OECs express all the components of the Nogo Receptor complex and that their migration is blocked by Myelin. Next, we used cell tracking and traction force microscopy to analyze OEC migration and its mechanical properties over Myelin. Our data relate the absence of traction force of OEC with lower migratory capacity, which correlates with changes in the F-Actin cytoskeleton and focal adhesion distribution. Lastly, OEC traction force and migratory capacity is enhanced after cell incubation with the Nogo Receptor inhibitor NEP1-40.
Mielina
Receptors cel·lulars
Neurobiologia
Myelin sheath
Cell receptors
Neurobiology
(c) Springer Verlag, 2012
Article
info:eu-repo/semantics/acceptedVersion
Springer Verlag
         

Show full item record

Related documents

Other documents of the same author

Carulla Martí, Patricia; Bribián Arruego, Ana; Rangel Rincones, Alejandra Helena; Gavín Marín, Rosalina; Ferrer, Isidro (Ferrer Abizanda); Caelles Franch, Carme; Río Fernández, José Antonio del; Llorens, Franc
Gil Fernández, Vanessa; Nocentini, Sara; Río Fernández, José Antonio del
Riggio, Cristina; Nocentini, Sara; Catalayud, María Pilar; Goya, Gerardo Fabian; Cuschieri, A. (Alfred), 1938-; Raffa, Vittoria; Río Fernández, José Antonio del
Bribián Arruego, Ana; Fontana, Xavier; Llorens, Franc; Gavín Marín, Rosalina; Reina del Pozo, Manuel; García-Verdugo, José Manuel; Torres, Juan María; Castro, Fernando de; Río Fernández, José Antonio del
Gil, Vanessa; Bichler, Zoe; Lee, Jae K.; Seira Oriach, Oscar; Llorens, Franc; Bribián Arruego, Ana; Morales, Ricardo; Claverol-Tinture, Enric; Soriano García, Eduardo; Sumoy, Lauro; Zheng, Binhai; Río Fernández, José Antonio del
 

Coordination

 

Supporters