Protein phosphatase inhibition assays for okadaic acid detection in shellfish: matrix effects, applicability and comparison with LC-MS/MS analysis

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RECERCAT Principal > Centres de Recerca de Catalunya (CERCA) > IRTA. Recerca i Tecnologia Agroalimentàries > A3 - Aqüicultura > Articles científics > Visualizar documento

Utilizad este identificador para citar o enlazar este documento: http://hdl.handle.net/2072/207214

dc.contributor	IRTA. Recerca i Tecnologia Agroalimentàries
dc.contributor.author	Garibo, Diana
dc.contributor.author	Dàmaso, Esther
dc.contributor.author	Eixarch Puigcerver, Helena
dc.contributor.author	De la Iglesia González, Pablo de la
dc.contributor.author	Fernández-Tejedor, Margarita
dc.contributor.author	Diogène Fadini, Jorge
dc.contributor.author	Pazos, Yolanda
dc.contributor.author	Campàs i Homs, Mònica
dc.date.accessioned	2013-02-22T12:31:42Z
dc.date.available	2013-02-22T12:31:42Z
dc.date.created	2012
dc.date.issued	2013-02-22
dc.identifier.citation	Garibo, D., et al. (2012). Protein phosphatase inhibition assays for okadaic acid detection in shellfish: matrix effects, applicability and comparison with LC-MS/MS analysis. Harmful Algae 19:68-75 http://dx.doi.org/10.1016/j.hal.2012.06.001
dc.identifier.uri	http://hdl.handle.net/2072/207214
dc.format.extent	35 p.
dc.language.iso	eng
dc.publisher	Elsevier
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.source	RECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.other	Toxines marines
dc.subject.other	Espectrometria de Mases
dc.subject.other	Cromatografia Líquida
dc.title	Protein phosphatase inhibition assays for okadaic acid detection in shellfish: matrix effects, applicability and comparison with LC-MS/MS analysis
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/draft
dc.embargo.terms	cap
dc.description.abstract	The applicability of the protein phosphatase inhibition assay (PPIA) to the determination of okadaic acid (OA) and its acyl derivatives in shellfish samples has been investigated, using a recombinant PP2A and a commercial one. Mediterranean mussel, wedge clam, Pacific oyster and flat oyster have been chosen as model species. Shellfish matrix loading limits for the PPIA have been established, according to the shellfish species and the enzyme source. A synergistic inhibitory effect has been observed in the presence of OA and shellfish matrix, which has been overcome by the application of a correction factor (0.48). Finally, Mediterranean mussel samples obtained from Rı´a de Arousa during a DSP closure associated to Dinophysis acuminata, determined as positive by the mouse bioassay, have been analysed with the PPIAs. The OA equivalent contents provided by the PPIAs correlate satisfactorily with those obtained by liquid chromatography–tandem mass spectrometry (LC–MS/MS).

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