Sistema de Salut de Catalunya
Institut Català de la Salut
[Carrillo-Rodriguez P] Proteomics Unit of University of Bergen (PROBE), University of Bergen, Bergen, Norway. Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. [Selheim F] Proteomics Unit of University of Bergen (PROBE), University of Bergen, Bergen, Norway. [Hernandez-Valladares M] Proteomics Unit of University of Bergen (PROBE), University of Bergen, Bergen, Norway. Department of Physical Chemistry, University of Granada, Granada, Spain. Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain
Vall d'Hebron Barcelona Hospital Campus
2023-03-01T11:48:36Z
2023-03-01T11:48:36Z
2023-01-16
Mass spectrometry; Proteomics; Workflows
Espectrometría de masas; Proteómica; Flujos de trabajo
Espectrometria de masses; Proteòmica; Fluxos de treball
The qualitative and quantitative evaluation of proteome changes that condition cancer development can be achieved with liquid chromatography–mass spectrometry (LC-MS). LC-MS-based proteomics strategies are carried out according to predesigned workflows that comprise several steps such as sample selection, sample processing including labeling, MS acquisition methods, statistical treatment, and bioinformatics to understand the biological meaning of the findings and set predictive classifiers. As the choice of best options might not be straightforward, we herein review and assess past and current proteomics approaches for the discovery of new cancer biomarkers. Moreover, we review major bioinformatics tools for interpreting and visualizing proteomics results and suggest the most popular machine learning techniques for the selection of predictive biomarkers. Finally, we consider the approximation of proteomics strategies for clinical diagnosis and prognosis by discussing current barriers and proposals to circumvent them.
This research was funded by the Research Council of Norway INFRASTRUKTUR-program (project number: 295910).
Article
Published version
English
Proteòmica; Càncer; Espectrometria de masses; ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Tandem Mass Spectrometry; DISEASES::Neoplasms; DISCIPLINES AND OCCUPATIONS::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Proteomics; TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::espectrometría de masas::espectrometría de masas en tándem; ENFERMEDADES::neoplasias; DISCIPLINAS Y OCUPACIONES::disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::bioquímica::proteómica
MDPI
Cancers;15(2)
https://doi.org/10.3390/cancers15020555
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
