dc.contributor.author |
García-Risco, Mario |
dc.contributor.author |
Calatayud, Sara |
dc.contributor.author |
Pedrini-Martha, Veronika |
dc.contributor.author |
Albalat, Ricard |
dc.contributor.author |
Dallinger, Reinhard |
dc.contributor.author |
Palacios Bonilla, Òscar |
dc.contributor.author |
Capdevila Vidal, Mercè |
dc.date |
2021 |
dc.identifier |
https://ddd.uab.cat/record/271827 |
dc.identifier |
urn:10.3390/ijms222313114 |
dc.identifier |
urn:oai:ddd.uab.cat:271827 |
dc.identifier |
urn:oai:egreta.uab.cat:publications/3559df8b-2a05-46a3-8626-961e1a81a12c |
dc.identifier |
urn:pure_id:146730506 |
dc.identifier |
urn:pmid:34884919 |
dc.identifier |
urn:pmcid:PMC8658704 |
dc.identifier |
urn:scopus_id:85120626091 |
dc.identifier |
urn:articleid:14220067v22n23p13114 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:8658704 |
dc.identifier |
urn:pmc-uid:8658704 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Ministerio de Economía y Competitividad BIO2015-67358-C2-2-P |
dc.relation |
Ministerio de Economía y Competitividad BIO2015-67358-C2-1-P |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-864 |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1665 |
dc.relation |
International journal of molecular sciences ; Vol. 22, Issue 23 (December 2021), art. 13114 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Biochemical classification |
dc.subject |
Cd-selective |
dc.subject |
Metallothioneins |
dc.subject |
Neritimorpha |
dc.title |
Metal-specificity divergence between Metallothioneins of Nerita peloronta (Neritimorpha, Gastropoda) sets the starting point for a novel chemical MT classification proposal |
dc.type |
Article |
dc.description.abstract |
Metallothioneins' (MTs) biological function has been a matter of debate since their discovery. The importance to categorize these cysteine-rich proteins with high coordinating capacity into a specific group led to numerous classification proposals. We proposed a classification based on their metal-binding abilities, gradually sorting them from those with high selectivity towards Zn/Cd to those that are Cu-specific. However, the study of the NpeMT1 and NpeMT2isoforms of Nerita peloronta, has put a new perspective on this classification. N. peloronta has been chosen as a representative mollusk to elucidate the metal-binding abilities of Neritimorpha MTs, an order without any MTs characterized recently. Both isoforms have been recombinantly synthesized in cultures supplemented with ZnII, CdII, or CuII, and the purified metal-MT complexes have been thoroughly characterized by spectroscopic and spectrometric methods, leading to results that confirmed that Neritimorpha share Cd-selective MTs with Caenogastropoda and Heterobranchia, solving a so far unresolved question. NpeMTs show high coordinating preferences towards divalent metal ions, although one of them (NpeMT1) shares features with the so-called genuine Zn-thioneins, while the other (NpeMT2) exhibits a higher preference for Cd. The dissimilarities between the two isoforms let a window open to a new proposal of chemical MT classification. |