Título:
|
Repositioning tolcapone as a potent inhibitor of transthyretin amyloidogenesis and associated cellular toxicity
|
Autor/a:
|
Sant'Anna, Ricardo; Gallego Alonso, Pablo; Robinson, Lei Z.; Pereira-Henriques, Alda; Ferreira, Nelson; Pinheiro, Francisca; Esperante, Sebastián; Pallarès i Goitiz, Irantzu; Huertas, Oscar; Almeida, Maria Rosário; Reixach, Natàlia; Insa, Raul; Velazquez-Campoy, Adrián; Reverter i Cendrós, David; Reig, Núria; Ventura, Salvador
|
Abstract:
|
Altres ajuts: S.V. has been granted an ICREA ACADEMIA award. SOM Biotech was funded by the Spanish Ministry of Health DIN 10-13 grant and the Catalan Competitiveness Agency FINEBT10-2-0037 grant. N. Reig and R.I. were funded by a Torres Quevedo grant by the Spanish Ministry of Economy and Competitiveness. |
Abstract:
|
Transthyretin (TTR) is a plasma homotetrameric protein implicated in fatal systemic amyloidoses. TTR tetramer dissociation precedes pathological TTR aggregation. Native state stabilizers are promising drugs to treat TTR amyloidoses. Here we repurpose tolcapone, an FDA-approved molecule for Parkinson's disease, as a potent TTR aggregation inhibitor. Tolcapone binds specifically to TTR in human plasma, stabilizes the native tetramer in vivo in mice and humans and inhibits TTR cytotoxicity. Crystal structures of tolcapone bound to wild-type TTR and to the V122I cardiomyopathy-associated variant show that it docks better into the TTR T pocket than tafamidis, so far the only drug on the market to treat TTR amyloidoses. These data indicate that tolcapone, already in clinical trials for familial amyloid polyneuropathy, is a strong candidate for therapeutic intervention in these diseases, including those affecting the central nervous system, for which no small-molecule therapy exists. |
Materia(s):
|
-Administration, Oral -Amyloid neuropathies, Familial -Animals -Benzophenones -Catechol O-methyltransferase inhibitors -Cell line -Dimerization -Drug repositioning -Healthy volunteers -Humans -Mice, Transgenic -Middle aged -Nitrophenols -Prealbumin -Protein aggregation, Pathological |
Derechos:
|
open access
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
https://creativecommons.org/licenses/by/4.0/ |
Tipo de documento:
|
Article |
Editor:
|
|
Compartir:
|
|
Uri:
|
https://ddd.uab.cat/record/225206
|