dc.contributor.author |
Rudilla, F. |
dc.contributor.author |
Gálvez-Montón, Carolina |
dc.contributor.author |
Gonzalo Calvo, David de |
dc.contributor.author |
Valero, Ana Gámez |
dc.contributor.author |
Gastelurrutia, Paloma |
dc.contributor.author |
Revuelta López, Elena |
dc.contributor.author |
Prat-Vidal, Cristina |
dc.contributor.author |
Soler Botija, Carolina |
dc.contributor.author |
Llucià Valldeperas, Aida |
dc.contributor.author |
Perea Gil, Isaac |
dc.contributor.author |
Iborra Egea, Oriol |
dc.contributor.author |
Borràs i Serres, Francesc Enric |
dc.contributor.author |
Lupón, Josep |
dc.contributor.author |
Llorente Cortés, Vicenta |
dc.contributor.author |
Bayés-Genís, Antoni |
dc.contributor.author |
Universitat Autònoma de Barcelona |
dc.date |
2017 |
dc.identifier |
https://ddd.uab.cat/record/186253 |
dc.identifier |
urn:10.1111/jcmm.13211 |
dc.identifier |
urn:oai:ddd.uab.cat:186253 |
dc.identifier |
urn:oai:egreta.uab.cat:publications/27e8df9d-0ad1-487d-9abc-54aa9d95e074 |
dc.identifier |
urn:pmid:28557183 |
dc.identifier |
urn:scopus_id:85019742607 |
dc.identifier |
urn:articleid:15824934v21p3000 |
dc.identifier |
urn:pmc-uid:5661250 |
dc.identifier |
urn:pmcid:PMC5661250 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:5661250 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Ministerio de Ciencia e Innovación SAF2014-59892-R |
dc.relation |
Ministerio de Economía y Competitividad JCI-2012-14025 |
dc.relation |
Instituto de Salud Carlos III FIS PI14-01682 |
dc.relation |
Instituto de Salud Carlos III FIS PI14-01729 |
dc.relation |
Instituto de Salud Carlos III CD14-00109 |
dc.relation |
Journal of Cellular and Molecular Medicine ; Vol. 21 (may 2017), p. 3000-3009 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Biomarker |
dc.subject |
Idiopathic dilated cardiomyopathy |
dc.subject |
Extracellular vesicles |
dc.subject |
Slrp1 |
dc.subject |
Size-exclusion chromatography |
dc.title |
Extracellular vesicles do not contribute to higher circulating levels of soluble 1 in idiopathic dilated cardiomyopathy |
dc.type |
Article |
dc.description.abstract |
Altres ajuts: Fundació La MARATÓ de TV3 (201502, 201516, 201521_10); Fundació Daniel Bravo Andreu; Sociedad Espanola de Cardiologıa; Societat Catalana de Cardiologia; Generalitat de Catalunya (SGR 2014, CERCA Programme); Fundació Bancaria La Caixa; Red de Terapia Celular TerCel (RD16/0011/0006); CIBER Cardiovascular (CB16/11/00403) |
dc.description.abstract |
Idiopathic dilated cardiomyopathy () is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low-density lipoprotein receptor-related protein 1 (1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels of soluble 1 (1) in patients and healthy controls and elucidated whether 1 is exported out of the myocardium through extracellular vesicles (s) to gain a better understanding of the pathogenesis of the disease. 1 α chain expression was analysed in samples collected from the left ventricles of explanted hearts using immunohistochemistry. 1 concentrations were determined in platelet-free plasma by enzyme-linked immunosorbent assay. Plasma-derived s were extracted by size-exclusion chromatography () and characterized by nanoparticle tracking analysis and cryo-transmission electron microscopy. The distributions of vesicular (9, 81) and myocardial (caveolin-3) proteins and 1 α chain were assessed in fractions by flow cytometry. 1 α chain was preferably localized to blood vessels in compared to control myocardium. Circulating 1 was increased in patients. 9- and 81-positive fractions enriched with membrane vesicles with the expected size and morphology were isolated from both groups. The 1 α chain was not present in these fractions, which were also positive for caveolin-3. The increase in circulating 1 in patients may be clinically valuable. Although s do not contribute to higher 1 levels in , a comprehensive analysis of content would provide further insights into the search for novel blood markers. |