Títol:
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Production of functional inclusion bodies in endotoxin-free Escherichia coli
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Autor/a:
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Rueda, Fabián; Cano-Garrido, Olivia; Mamat, Uwe; Wilke, Kathleen; Seras-Franzoso, Joaquin; Garcia-Fruitos, Elena; Villaverde Corrales, Antonio
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Abstract:
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Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a relatively pure source of protein for in vitro refolding, IBs are under exploration as functional, protein-releasing materials in regenerative medicine and protein replacement therapies. Endotoxin removal is a critical step for downstream applications of therapeutic proteins. The same holds true for IBs as they are often highly contaminated with cell-wall components of the host cells. Here, we have investigated the production of IBs in a recently developed endotoxin-free E. coli strain. The characterization of IBs revealed this mutant as a very useful cell factory for the production of functional endotoxin-free IBs that are suitable for the use at biological interfaces without inducing endotoxic responses in human immune cells |
Matèries:
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-E. coli -Recombinant protein -Inclusion bodies -Endotoxin -LPS -LPS-free |
Drets:
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open access
Tots els drets reservats.
https://rightsstatements.org/vocab/InC/1.0/ |
Tipus de document:
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Article |
Publicat per:
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Compartir:
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Uri:
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https://ddd.uab.cat/record/132797
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