dc.contributor.author |
Vilahur Chiaraviglio, Nadia, 1982- |
dc.contributor.author |
Baccarelli, Andrea A. |
dc.contributor.author |
Bustamante Pineda, Mariona |
dc.contributor.author |
Agramunt, Silvia |
dc.contributor.author |
Byun, Hyang-Min |
dc.contributor.author |
Fernandez, Mariana F. |
dc.contributor.author |
Sunyer Deu, Jordi |
dc.contributor.author |
Estivill, Xavier, 1955- |
dc.date |
2013 |
dc.identifier.citation |
Vilahur N, Baccarelli AA, Bustamante M, Agramunt S, Byun HM, Fernandez MF et al. Storage conditions and stability of global DNA methylation in placental tissue. Epigenomics. 2013 Jun; 5(3): 341-8. DOI: 10.2217/epi.13.29 |
dc.identifier.citation |
1750-1911 |
dc.identifier.citation |
http://dx.doi.org/10.2217/epi.13.29 |
dc.identifier.uri |
http://hdl.handle.net/10230/36592 |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Future Medicine |
dc.rights |
© 2013 Future Medicine Ltd |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
ADN -- Metabolisme |
dc.subject |
ADN -- Metilació |
dc.subject |
Placenta -- Metabolisme |
dc.title |
Storage conditions and stability of global DNA methylation in placental tissue |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/acceptedVersion |
dc.description.abstract |
AIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30 min, 1 h, 2 h, 6 h and 24 h. Global DNA methylation was measured by bisulfite pyrosequencing of repetitive elements and the luminometric methylation assay. RESULTS: Small changes in global DNA methylation in relation to time-to-storage were observed by pyrosequencing, with a coefficient of variation (COV) of 2.49% (placenta 1) and 2.86% (placenta 2), similar to the mean technical variation observed for pyrosequencing (COV: 1.91 and 1.51%, respectively). A luminometric methylation assay yielded more variable results in the two placentas analyzed, both among time points (COV: 9.13 and 10.35%, respectively) and technical replicates (COV: 11.60 and 9.80%, respectively). CONCLUSION: Global DNA methylation is stable at room temperature. However, some techniques to measure methylation might be confounded by DNA degradation caused by a delay in storage. |
dc.description.abstract |
This study was funded by grants from the Spanish Ministry of Health (FIS-PI041436 and PI11/00610), Instituto de Salud Carlos III (Red Infancia y MedioAmbiente G03/176 and CB06/02/0041) and the Generalitat de Catalunya-Comissió Interdepartamental de Recerca i Innovació Tecnològica 1999SGR 00241. AA Baccarelli receives support from the Harvard School of Public Health and National Institute of Environmental Health Sciences Center for Environmental Health (ES000002). N Vilahur was supported by a Formación de Personal Investigador Grant from the Spanish Ministry of Health and a Formación de Personal Investigador Grant for Short Research Stays in Foreign Institutions (BES-2009-023933) and MF Fernandez by the Ramon y Cajal Research Grant from the Spanish Ministry of Education |