Sulfate metabolites as alternative markers for the detection of 4-chlorometandienone misuse in doping control

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dc.contributor.author	Balcells Aribau, Georgina, 1987-
dc.contributor.author	Gómez, Cristina
dc.contributor.author	Garrostas, Lorena
dc.contributor.author	Pozo Mendoza, Óscar J., 1975-
dc.contributor.author	Ventura Alemany, Rosa
dc.date	2016
dc.identifier.citation	Balcells G, Gómez C, Garrostas L, Pozo ÓJ, Ventura R. Sulfate metabolites as alternative markers for the detection of 4-chlorometandienone misuse in doping control. Drug Test Anal. 2016 Sep 30. DOI: 10.1002/dta.2101.
dc.identifier.citation	1942-7603
dc.identifier.citation	http://dx.doi.org/10.1002/dta.2101
dc.identifier.uri	http://hdl.handle.net/10230/32509
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Wiley
dc.relation	Drug Testing and Analysis. 2017;9(7):983-93.
dc.relation	info:eu-repo/grantAgreement/ES/3PN/DEP2012-35612
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.rights	This is the peer reviewed version of the following article: Balcells G, Gómez C, Garrostas L, Pozo ÓJ, Ventura R. Sulfate metabolites as alternative markers for the detection of 4-chlorometandienone misuse in doping control. Drug Test Anal. 2016 Sep 30. [Epub ahead of print] , which has been published in final form at http://dx.doi.org/10.1002/dta.2101. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
dc.subject	Dopatge en els esports -- Control
dc.subject	Metabòlits
dc.subject	Sulfats
dc.title	Sulfate metabolites as alternative markers for the detection of 4-chlorometandienone misuse in doping control
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/acceptedVersion
dc.description.abstract	Sulfate metabolites have been described as long-term metabolites for some anabolic androgenic steroids (AAS). 4-chlorometandienone (4Cl-MTD) is one of the most frequently detected AAS in sports drug testing and it is commonly detected by monitoring metabolites excreted free or conjugated with glucuronic acid. Sulfation reactions of 4Cl-MTD have not been studied. The aim of this work was to evaluate the sulfate fraction of 4Cl-MTD metabolism by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to establish potential long-term metabolites valuable for doping control purposes. 4Cl-MTD was administered to two healthy male volunteers and urine samples were collected up to 8 days after administration. A theoretical selected reaction monitoring (SRM) method working in negative mode was developed. Ion transitions were based on ionization and fragmentation behaviour of sulfate metabolites as well as specific neutral losses (NL of 15 Da and NL of 36 Da) of compounds with related chemical structure. Six sulfate metabolites were detected after the analysis of excretion study samples. Three of the identified metabolites were characterized by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Results showed that five out of the six identified sulfate metabolites were detected in urine up to the last collected samples from both excretion studies. Copyright © 2016 John Wiley & Sons, Ltd.
dc.description.abstract	The financial support received from the World Anti-Doping Agency WADA (12A21RV), Ministerio de Economía y Competividad (Gobierno de España) (Project number DEP2012-35612) and Generalitat de Catalunya (Consell Català de l’Esport, DIUE 2014SGR 692) is gratefully acknowledged

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