| dc.contributor.author |
Nikkhahi, Farhad |
| dc.contributor.author |
Dallal, Mohammad Mehdi Soltan |
| dc.contributor.author |
Alimohammadi, Mahmood |
| dc.contributor.author |
Foroushani, Abbas Rahimi |
| dc.contributor.author |
Rajabi, Zahra |
| dc.contributor.author |
Fardsanei, Fatemeh |
| dc.contributor.author |
Imeni, Seyed Mostafa |
| dc.contributor.author |
Bonab, Parisa Torabi |
| dc.date |
2017 |
| dc.identifier |
Nikkhahi, F., Dallal, M. M. S., Alimohammadi, M., Foroushani, A. R., Rajabi, Z., Fardsanei, F., et al. (2017). Phage therapy: Assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by salmonella enteritidis in mice. Gastroenterology and Hepatology from Bed to Bench, 10(2), 131-136. |
| dc.identifier |
2008-4234 |
| dc.identifier |
http://hdl.handle.net/10854/5021 |
| dc.identifier |
https://doi.org/10.22037/ghfbb.v0i0.1003 |
| dc.identifier.uri |
http://hdl.handle.net/10854/5021 |
| dc.description |
Aim: This work aims to isolate and perform comparative studies of a phages active against a Salmonella enteritidis strain from Iran. Also, suitable phage candidates for therapy of mice will be selected.
Background: Bacteriophage is of particular interest as a biocontrol agent in the prevention of food-borne illnesses. In recent years tend to use bacteriophages to control pathogenic bacteria has increased. A bacteriophage is considered to be a potent antibiotic alternative for treating bacterial infections.
Methods: the specific phages against Salmonella Enteritidis was isolated and candidates for therapy of mice will be selected. Mouses divided into the six specific groups. Groups of mice were as follows: A: Bacteri (control) B: Bacteri+ bacteriophage (Simultaneous), C: Bacteri + bacteriophage Four days later, D: Bacteriophage + bacteri four days later E: Bacteri+ Ciprofloxacin (Simultaneous) F: Bacteri+ ciprofloxacin+ bacteriophage (Simultaneous).
Results: In this study, a lytic bacteriophage is isolated and it shows that phage has a head size of 46 nm and without a tail, by using an electron microscope. Oral administration of a single dose of 2 × 109 PFU/mouse bacteriophage enable to protect mouse against salmonellosis and it causes treatment of salmonellosis in mice.
Conclusion: The use of this phage compared to ciprofloxacin shows that in addition of the treatment of mouse, it also prevents weight loss. |
| dc.format |
application/pdf |
| dc.language |
eng |
| dc.publisher |
Research Institute for Gastroenterology and Liver Diseases |
| dc.rights |
Aquest document està subjecte a aquesta llicència Creative Commons |
| dc.rights |
http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights |
info:eu-repo/semantics/openAccess |
| dc.subject |
Bacteriòfags |
| dc.subject |
Salmonel·la |
| dc.title |
Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice |
| dc.type |
info:eu-repo/semantics/article |
| dc.type |
info:eu-repo/publishedVersion |
