|
Title:
|
Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity
|
|
Author:
|
Mota Martorell, Natalia; Jové Font, Mariona; Pradas Barriga, Irene; Sanchez, Isabel; Gómez, José; Naudí i Farré, Alba; Barja, Gustavo; Pamplona Gras, Reinald
|
|
Notes:
|
Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.
This work was supported by the Spanish Ministry of Economy and Competitiveness, Institute of Health Carlos III (grant number PI14/00328), the Spanish Ministry of Science, Innovation and Universities (RTI2018-099200-B-I00), and the Generalitat of Catalonia, Agency for Management of University and Research Grants (2017SGR696) and Department of Health (SLT002/16/00250) to R.P, and PR [19] BIO MET 0155 to GB.This study has been co-financed by FEDER funds from the European Union (“A way to build Europe”). IRBLleida is a CERCA Programme/Generalitat of Catalonia. |
|
Subject(s):
|
-Complex I
-Droplet digital PCR
-Longevity
-Mammals
-Mitochondria
-NDUFV2 subunit
-NDUFS4 subunit
-VDAC
-Western blot |
|
Rights:
|
cc-by-nc-nd (c) Mota Martorell et al., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
|
|
Document type:
|
Article
Article - Published version |
|
Published by:
|
Elsevier
|
|
Share:
|
|
