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Título:
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Cross-species infectivity of H3N8 influenza virus in an experimental infection in swine
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Autor/a:
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Solórzano, Alicia; Foni, Emanuela; Córdoba, Lorena; Baratelli, Massimiliano; Razzuoli, Elisabetta; Bilato, Dania; Martín del Burgo, María Ángeles; Perlin, David S.; Martínez, Jorge; Martínez-Orellana, Pamela; Fraile Sauce, Lorenzo José; Chiapponi, Chiara; Amadori, Massimo; Del Real, Gustavo; Montoya, Maria
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Notas:
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Avian influenza A viruses have gained increasing attention due to their ability to cross the species barrier and cause severe disease
in humans and other mammal species as pigs. H3 and particularly H3N8 viruses, are highly adaptive since they are found in
multiple avian and mammal hosts. H3N8 viruses have not been isolated yet from humans; however, a recent report showed that
equine influenza A viruses (IAVs) can be isolated from pigs, although an established infection has not been observed thus far in
this host. To gain insight into the possibility of H3N8 avian IAVs to cross the species barrier into pigs, in vitro experiments and
an experimental infection in pigs with four H3N8 viruses from different origins (equine, canine, avian, and seal) were performed.
As a positive control, an H3N2 swine influenza virus A was used. Although equine and canine viruses hardly replicated
in the respiratory systems of pigs, avian and seal viruses replicated substantially and caused detectable lesions in inoculated pigs
without previous adaptation. Interestingly, antibodies against hemagglutinin could not be detected after infection by hemagglutination
inhibition (HAI) test with avian and seal viruses. This phenomenon was observed not only in pigs but also in mice immunized
with the same virus strains. Our data indicated that H3N8 IAVs from wild aquatic birds have the potential to cross the
species barrier and establish successful infections in pigs that might spread unnoticed using the HAI test as diagnostic tool.
We thank Jaime Maldonado and HIPRA (Spain) for the A/Swine/Spain/
54008/2004 (H3N2) strain, Edward J. Dubovi and Cornell University for
the A/Canine/NY/105447/08 (H3N8) IAV strain, T. M. Chambers and the
University of Kentucky for the A/Equine/OH/1/03 (H3N8) IAV strain,
and Hon Ip and the U.S. Geological Survey National Wildlife Health
Center for the A/American black duck/Maine/44411-532/2008 (H3N8)
and the A/Harbor Seal/New Hampshire/179629/2011 (H3N8) IAV
strains. We thank Sergio López, David Solanes, Francisco X. Abad, Jordi
Alberola, Jaume Martorell, and Eduard J. Cunilleras for help in providing
different samples and during the experimental infections, as well as the
personnel in Cat3 laboratories and the animal house. We thank Adolfo
García-Sastre for providing materials and for support as the principal
investigator of the NIAID-funded Center for Research in Influenza Pathogenesis
(HHSN266200700010C).
The research leading to these results received funding from the European
Community’s Seventh Framework Programme (FP7, 2007-2013),
the Research Infrastructures Action under grant FP7-228393 (a NADIR
project), and projects AGL2010-22200-C02-01 and AGL2007-60274 of
the Spanish Ministry of Science and Innovation. |
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Materia(s):
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-Porcs--Infeccions
-Animals--Malalties
-Porcs--Virus |
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Derechos:
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(c) American Society for Microbiology, 2015
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Tipo de documento:
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Artículo
Artículo - Versión aceptada |
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Editor:
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American Society for Microbiology
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