dc.contributor.author |
Torres Sanchis, Rosario |
dc.contributor.author |
Solsona Aixalà, Cristina |
dc.contributor.author |
Viñas Almenar, Inmaculada |
dc.contributor.author |
Usall i Rodié, Josep |
dc.contributor.author |
Plaza Portolés, Ma. Pilar |
dc.contributor.author |
Teixidó i Espasa, Neus |
dc.date |
2017-05-23T08:18:55Z |
dc.date |
2014 |
dc.date |
2017-05-23T08:18:58Z |
dc.date |
10000-01-01 |
dc.identifier |
1364-5072 |
dc.identifier |
http://hdl.handle.net/10459.1/59681 |
dc.identifier |
https://doi.org/10.1111/jam.12511 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/59681 |
dc.description |
AIMS: This study aimed to evaluate different packaging strategies to extend the shelf life of a freeze-dried formulation of the biocontrol agent Pantoea agglomerans strain CPA-2. METHODS AND RESULTS: Different materials and atmosphere packaging conditions (vacuum and air) were analysed on formulated P. agglomerans cells stored at 25, 5 and -20°C. Results showed the viability of CPA-2 cells stored at 5 or -20°C was significantly higher than when stored at 25°C. The highest viabilities were observed with the plastic material designated as Bottle 1, in nonvacuum packaging in all storage temperatures: 50% after 3 months at 25°C, 100% after 8 months at 5°C and 100 and 74% after 12 and 18 months, respectively, at -20°C; the final concentration was 10(12) CFU g(-1), a good concentration for a commercial product. The efficacy to control blue and green mould on apples and oranges, respectively, of these packed and stored cells was similar to fresh CPA-2 cells. CONCLUSIONS: This work showed a suitable packaging strategy for a freeze-dried formulation of the CPA-2, providing a good shelf life and efficacy against the major postharvest diseases of apples and citrus based on a plastic bottle stored at cold or frozen storage conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: The last phase of the commercial development process for biocontrol agents is presented in this work. A bacterium-based product that ensures the efficacy, stability and easy application of the antagonist to control postharvest fungal diseases on fruit was successfully obtained. |
dc.description |
This research was supported by the national project RTA2009-00053 (Plan Nacional de I
+ D Ministerio de Ciencia e Innovación, Spanish Government) and by a ‘Ramon y Cajal’ contract (R.T.). Authors are also grateful with the company BIODURCAL S.L. for providing some of the plastic bags used in this work. |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
Society for Applied Microbiology |
dc.relation |
MICINN/PN2008-2011/RTA2009-0005 |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.1111/jam.12511 |
dc.relation |
Journal of Applied Microbiology, 2014, vol. 117, núm. 1, p. 173-184 |
dc.rights |
(c) Society for Applied Microbiology, 2014 |
dc.rights |
info:eu-repo/semantics/restrictedAccess |
dc.subject |
Apples |
dc.subject |
Biological control |
dc.subject |
Oranges |
dc.subject |
Packaging conditions |
dc.title |
Optimization of packaging and storage conditions of a freeze-dried Pantoea agglomerans formulations for controlling postharvest diseases in fruit |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |