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Title:
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Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
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Author:
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Valls i Bautista, Cristina; Piñol Felis, Carme; Reñé Espinet, Josep Maria; Buenestado Garcia, Juan; Viñas Salas, Joan
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Notes:
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Background: colorectal cancer is the third cancer cause of death
in Spain. It is important to investigate new tumoral markers for early
diagnosis, disease monitoring and prevention strategies. Telomeres
protect the chromosome from degradation by nucleases and endto-
end fusion. The progressive loss of the telomeric ends of chromosomes
is an important mechanism in the timing of human cellular
aging. Telomeric Repeat Factor 1 (TRF1) is a protein that binds at
telomere ends.
Purpose: to measure the concentrations of TRF1 and the relationships
among telomere length, telomerase activity, and TRF1
levels in tumor and normal colorectal mucosa.
Method: from normal and tumoral samples of 83 patients who
underwent surgery for colorectal cancer we analyzed TRF1 protein
concentration by Western Blot, telomerase activity, by the fluorescent-
telomeric repeat amplification protocol assay and telomere
length by Southern Blot.
Results: high levels of TRF1 were observed in 68.7% of tumor
samples, while the majority of normal samples (59%) showed negative
or weak TRF1 concentrations. Among the tumor samples,
telomere length was significantly associated with TRF1 protein levels
(p = 0.023).
Conclusions: a relationship was found between telomere length
and TRF1 abundance protein in tumor samples, which means that
TRF1 is an important factor in the tumor progression and maybe
a diagnostic factor. |
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Subject(s):
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-Telomeric repeat factor 1
-Telomere length
-Colorectal cancer
-Telomerase activity |
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Rights:
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(c) Arán Ediciones, 2012
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Document type:
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article
publishedVersion |
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Published by:
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Sociedad Española de Patología Digestiva;
Arán Ediciones
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