To access the full text documents, please follow this link: http://hdl.handle.net/10459.1/48093

(R)-roscovitine (CYC202, Seliciclib) sensitizes SH-SY5Y neuroblastorna cells to nutlin-3-induced apoptosis
Ribas i Fortuny, Judit; Boix Torras, Jacint; Meijer, Laurent
In this study, we have analyzed the consequences, on several neuroblastoma cell lines, of combined treatments with (R)-roscovitine (CYC202, Seliciclib), a CDK inhibitory drug, and nutlin-3, a p53 activating drug. Both compounds were found to synergize, causing significant levels of apoptosis in cultured cells when combined at sublethal concentrations. In SH-SY5Y cells, Bcl-XL protein overexpression protected from apoptosis induced by either nutlin-3 alone or the (R)-roscovitine plus nutlin-3 association but failed to prevent apoptosis triggered by (R)-roscovitine alone. Moreover, Western blot studies showed that (R)-roscovitine increased nutlin-3-mediated p53 stabilization. Therefore, we conclude the contribution of (R)-roscovitine to the synergism is basically the sensitization of SH-SY5Y cells to the action of nutlin-3 on p53. The relevance of this pharmacological synergism with respect to the treatment of neuroblastoma is discussed.
-Apoptosis
-Cell Cycle
-Nutlins
-p53
-Roscovitine
-Apoptosi
-Càncer
-Cicle cel·lular
-Apoptosis
-Cancer
-Cell cycle
(c) Elsevier, 2006
Article
Article - Accepted version
Elsevier
         

Full text files in this document

Files Size Format View
009195.pdf 3.831 MB application/pdf View/Open

Show full item record

Related documents

Other documents of the same author

 

Coordination

 

Supporters