| Title: | Metal-specificity divergence between Metallothioneins of Nerita peloronta (Neritimorpha, Gastropoda) sets the starting point for a novel chemical MT classification proposal |
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| Author: | García-Risco, Mario; Calatayud, Sara; Pedrini-Martha, Veronika; Albalat, Ricard; Dallinger, Reinhard; Palacios Bonilla, Òscar; Capdevila Vidal, Mercè |
| Abstract: | Metallothioneins' (MTs) biological function has been a matter of debate since their discovery. The importance to categorize these cysteine-rich proteins with high coordinating capacity into a specific group led to numerous classification proposals. We proposed a classification based on their metal-binding abilities, gradually sorting them from those with high selectivity towards Zn/Cd to those that are Cu-specific. However, the study of the NpeMT1 and NpeMT2isoforms of Nerita peloronta, has put a new perspective on this classification. N. peloronta has been chosen as a representative mollusk to elucidate the metal-binding abilities of Neritimorpha MTs, an order without any MTs characterized recently. Both isoforms have been recombinantly synthesized in cultures supplemented with ZnII, CdII, or CuII, and the purified metal-MT complexes have been thoroughly characterized by spectroscopic and spectrometric methods, leading to results that confirmed that Neritimorpha share Cd-selective MTs with Caenogastropoda and Heterobranchia, solving a so far unresolved question. NpeMTs show high coordinating preferences towards divalent metal ions, although one of them (NpeMT1) shares features with the so-called genuine Zn-thioneins, while the other (NpeMT2) exhibits a higher preference for Cd. The dissimilarities between the two isoforms let a window open to a new proposal of chemical MT classification. |
| Subject(s): | -Biochemical classification -Cd-selective -Metallothioneins -Neritimorpha |
| Rights: | open access
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| Document type: | Article |
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| Uri: | https://ddd.uab.cat/record/271827 |
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