dc.contributor.author |
Tang, Xiaoqian |
dc.contributor.author |
Zhang, Qi |
dc.contributor.author |
Pividori, María Isabel |
dc.contributor.author |
Zhang, Zhaowei |
dc.contributor.author |
Marty, Jean-Louis |
dc.contributor.author |
Catanante, Gaëlle |
dc.date |
2022 |
dc.identifier |
https://ddd.uab.cat/record/256090 |
dc.identifier |
urn:10.3390/bios12020059 |
dc.identifier |
urn:oai:ddd.uab.cat:256090 |
dc.identifier |
urn:pmcid:PMC8869234 |
dc.identifier |
urn:pmc-uid:8869234 |
dc.identifier |
urn:pmid:35200320 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:8869234 |
dc.identifier |
urn:articleid:20796374v12n2p59 |
dc.identifier |
urn:oai:egreta.uab.cat:publications/75c8c383-6d39-4ab0-9adf-67c3ee6cf799 |
dc.identifier |
urn:scopus_id:85123917580 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Agencia Estatal de Investigación PID2019-106625RB-I00 |
dc.relation |
Biosensors ; Vol. 12, Issue 2 (January 2022), art. 59 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Patulin |
dc.subject |
Aptamer |
dc.subject |
Lateral flow assay |
dc.subject |
On-site |
dc.subject |
Apple juice |
dc.title |
A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice |
dc.type |
Article |
dc.description.abstract |
Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B (AFB), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice. |