| Title: | Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy |
|---|---|
| Author: | Guillén-Gómez, Elena; Bardají de Quixano, Beatriz; Ferrer, Sílvia; Brotons, Carlos; Knepper, Mark A.; Carrascal Pérez, Montserrat; Abian, Joaquin; Mas, José M.; Calero, Francesca; Ballarín Castan, José Aurelio; Fernandez-Llama, Patricia; Universitat Autònoma de Barcelona |
| Abstract: | Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (n = 9) and without (n = 11) incipient DN (microalbuminuria). Sample collection included blood and urine at baseline (control and DN basal) and, in DN patients, after 3 months of losartan treatment (DN treated). Urinary proteome analysis identified 166 differentially abundant proteins between controls and DN patients, 27 comparing DN-treated and DN-basal patients, and 182 between DN-treated patients and controls. The mathematical modeling analysis predicted 80 key proteins involved in DN pathophysiology and 15 in losartan effect, a total of 95 proteins. Out of these 95, 7 are involved in both processes. VCAM-1 and neprilysin stand out of these 7 for being differentially expressed in the urinary proteome. We observed an increase of VCAM-1 urine levels in DN-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria; the latter was confirmed by ELISA. Our results point to neprilysin and VCAM-1 as potential candidates in DN pathology and treatment. |
| Rights: | open access
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ |
| Document type: | Article |
| Published by: | |
| Share: |
|
| Uri: | https://ddd.uab.cat/record/253541 |
Related documents
Other documents of the same author
Guillén-Gómez, Elena; Silva, Irene; Serra, Núria; Caballero, Francisco; Leal, Jesús; Breda, Alberto; San Martín, Rody; Pastor-Anglada, Marçal; Ballarín Castan, José Aurelio; Guirado, Luis; Díaz Encarnación, Montserrat Mercedes; Universitat Autònoma de Barcelona
Jiménez-Alesanco, A.; Marcuello, M.; Pastor-Jiménez, M.; López-Puerto, L.; Bonjoch, Laia; Gironella, Meritxell; Carrascal Pérez, Montserrat; Abian, Joaquin; de Madaria, Enrique; Closa Autet, Daniel; Universitat Autònoma de Barcelona
Chauvigné, François; Ducado, Carla; Ferré, Alba; Hansen, Tom; Carrascal Pérez, Montserrat; Abian, Joaquin; Finn, Roderick Nigel; Cerdà, Joan
Gella, Alejandro; Prada-Dacasa, Patricia; Carrascal Pérez, Montserrat; Urpi, Andrea; González-Torres, Melania; Abian, Joaquin; Sanz, Elisenda; Quintana Romero, Albert
González Sepúlveda, Marta; Yusof Omar, Muhammad; Hamdon, Sally; Ma, Guofen; Rosell-Vilar, Santi; Raivio, Noora; Abass, Doaa; Martínez-Rivas, Anna; Vila Bover, Miquel; Giraldo, Jesús; Carrascal Pérez, Montserrat; Abian, Joaquin; Gil, Carles; Sabriá, Josefa; Ortiz de Pablo, Jordi; Moreno-Delgado, David