dc.contributor.author |
Vandelli, Andrea |
dc.contributor.author |
Monti, Michele |
dc.contributor.author |
Milanetti, Edoardo |
dc.contributor.author |
Armaos, Alexandros |
dc.contributor.author |
Rupert, Jakob |
dc.contributor.author |
Zacco, Elsa |
dc.contributor.author |
Bechara, Elias |
dc.contributor.author |
Delli Ponti, Riccardo |
dc.contributor.author |
Tartaglia, Gian Gaetano |
dc.date |
2020 |
dc.date.accessioned |
2022-10-29T15:02:46Z |
dc.date.available |
2022-10-29T15:02:46Z |
dc.date.issued |
2022-10-29 |
dc.identifier |
https://ddd.uab.cat/record/238605 |
dc.identifier |
urn:10.1093/nar/gkaa864 |
dc.identifier |
urn:oai:ddd.uab.cat:238605 |
dc.identifier |
urn:pmcid:PMC7672441 |
dc.identifier |
urn:pmc-uid:7672441 |
dc.identifier |
urn:pmid:33068416 |
dc.identifier |
urn:articleid:13624962v48n20p11270 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:7672441 |
dc.identifier.uri |
http://hdl.handle.net/2072/523650 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
European Commission 309545 |
dc.relation |
European Commission 855923 |
dc.relation |
European Commission 727658 |
dc.relation |
European Commission 825070 |
dc.relation |
Agencia Estatal de Investigación BFU2017-86970-P |
dc.relation |
Nucleic acids research ; Vol. 48, Issue 20 (November 2020), p. 11270-11283 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by-nc/4.0/ |
dc.title |
Structural analysis of SARS-CoV-2 genome and predictions of the human interactome |
dc.type |
Article |
dc.description.abstract |
Specific elements of viral genomes regulate interactions within host cells. Here, we calculated the secondary structure content of >2000 coronaviruses and computed >100 000 human protein interactions with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The genomic regions display different degrees of conservation. SARS-CoV-2 domain encompassing nucleotides 22 500-23 000 is conserved both at the sequence and structural level. The regions upstream and downstream, however, vary significantly. This part of the viral sequence codes for the Spike S protein that interacts with the human receptor angiotensin-converting enzyme 2 (ACE2). Thus, variability of Spike S is connected to different levels of viral entry in human cells within the population. Our predictions indicate that the 5' end of SARS-CoV-2 is highly structured and interacts with several human proteins. The binding proteins are involved in viral RNA processing, include double-stranded RNA specific editases and ATP-dependent RNA-helicases and have strong propensity to form stress granules and phase-separated assemblies. We propose that these proteins, also implicated in viral infections such as HIV, are selectively recruited by SARS-CoV-2 genome to alter transcriptional and post-transcriptional regulation of host cells and to promote viral replication. |