Título:
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Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
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Autor/a:
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Dopico, Eva; Del-Rei, Rodrigo Pimenta; Espinoza, Bertha; Ubillos, Itziar; Zanchin, Nilson Ivo Tonin; Sulleiro Igual, Elena; Moure García, Zaira; Celedon, Paola Alejandra Fiorani; Souza, Wayner Vieira; da Silva, Edimilson Domingos; Gomes, Yara Miranda; Santos, Fred Luciano Neves; Universitat Autònoma de Barcelona
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Abstract:
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Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi -infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in non-endemic settings. |
Materia(s):
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-Chagas disease -Trypanosoma cruzi -Chimeric antigens -Immunoassay -Accuracy |
Derechos:
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open access
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Article |
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Uri:
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https://ddd.uab.cat/record/226393
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