dc.contributor.author |
Gambato, Martina |
dc.contributor.author |
Gregori i Font, Josep |
dc.contributor.author |
Quer, Josep |
dc.contributor.author |
Koutsoudakis, George |
dc.contributor.author |
González, Patricia |
dc.contributor.author |
Caro-Pérez, Noelia |
dc.contributor.author |
Garcia-Cehic, D. |
dc.contributor.author |
García-González, Neris |
dc.contributor.author |
González-Candelas, Fernando |
dc.contributor.author |
Ignacio Esteban, Juan |
dc.contributor.author |
Crespo, Gonzalo |
dc.contributor.author |
Navasa, Miquel |
dc.contributor.author |
Forns, Xavier |
dc.contributor.author |
Pérez-Del-Pulgar, Sofía |
dc.date |
2019 |
dc.identifier |
https://ddd.uab.cat/record/223341 |
dc.identifier |
urn:10.1099/jgv.0.001175 |
dc.identifier |
urn:oai:ddd.uab.cat:223341 |
dc.identifier |
urn:scopus_id:85059428161 |
dc.identifier |
urn:articleid:14652099v100n1p63 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017_SGR_1753 |
dc.relation |
Ministerio de Economía y Competitividad BFU2017-89594R |
dc.relation |
Instituto de Salud Carlos III PI16/ 00337 |
dc.relation |
Instituto de Salud Carlos III PI15/00151 |
dc.relation |
Instituto de Salud Carlos III PI13/00155 |
dc.relation |
The Journal of general virology ; Vol. 100 Núm. 1 (january 2019), p. 63-68 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Hepatitis C virus |
dc.subject |
Liver transplantation |
dc.subject |
Cholestatic hepatitis C |
dc.subject |
Quasispecies |
dc.subject |
Deep sequencing |
dc.title |
Hepatitis C virus intrinsic molecular determinants may contribute to the development of cholestatic hepatitis after liver transplantation |
dc.type |
Article |
dc.description.abstract |
Cholestatic hepatitis C (CHC) is a severe form of hepatitis C virus (HCV) infection recurrence that leads to high graft loss rates early after liver transplantation (LT). To investigate the pathogenic mechanisms of CHC, we analysed HCV quasispecies in CHC patients compared to a control group (mild hepatitis C recurrence) by deep pyrosequencing. At the time of LT, NS5B quasispecies complexity was similar between the two groups but, after LT, it decreased more sharply in CHC patients than in the control group. Interestingly, the major variant before LT propagated efficiently and remained as the dominant sequence after LT in 62% of CHC patients versus 11% of controls (P=0.031). Sequence analysis of the complete nonstructural region in a limited number of patients revealed a potential 12 aa signature specific to the CHC group. These data suggest that intrinsic molecular determinants in the circulating HCV quasispecies may provide a fitness advantage, contributing to the development of CHC. |