dc.contributor.author |
Pesarrodona Roches, Mireia |
dc.contributor.author |
Sánchez-García, Laura |
dc.contributor.author |
Seras-Franzoso, Joaquin |
dc.contributor.author |
Sánchez Chardi, Alejandro |
dc.contributor.author |
Baltà Foix, Ricardo |
dc.contributor.author |
Cámara-Sánchez, Patricia |
dc.contributor.author |
Gener, Petra |
dc.contributor.author |
Jara, José Juan |
dc.contributor.author |
Pulido, Daniel |
dc.contributor.author |
Serna, Naroa |
dc.contributor.author |
Schwartz, Simó |
dc.contributor.author |
Royo, Miriam |
dc.contributor.author |
Villaverde Corrales, Antonio |
dc.contributor.author |
Abasolo, Ibane |
dc.contributor.author |
Vázquez Gómez, Esther |
dc.contributor.author |
Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia |
dc.date |
2020 |
dc.identifier |
https://ddd.uab.cat/record/233717 |
dc.identifier |
urn:10.1021/acsami.9b15803 |
dc.identifier |
urn:oai:ddd.uab.cat:233717 |
dc.identifier |
urn:scopus_id:85078916339 |
dc.identifier |
urn:articleid:19448252v12n5p5381 |
dc.identifier |
urn:oai:egreta.uab.cat:publications/52849660-5cf4-4cf6-9e28-151abaa18435 |
dc.identifier |
urn:pmid:31840972 |
dc.format |
application/pdf |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Instituto de Salud Carlos III PI18/00871 |
dc.relation |
Instituto de Salud Carlos III PI15/00272 |
dc.relation |
Instituto de Salud Carlos III PI17/02242 |
dc.relation |
Ministerio de Economía y Competitividad BIO2016-76063-R |
dc.relation |
Ministerio de Economía y Competitividad SAF2014-60138-R |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1439 |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 |
dc.relation |
Agència de Gestió d'Ajuts Universitaris i de Recerca 2018FI_B2_00051 |
dc.relation |
ACS applied materials & interfaces ; Vol. 12, Issue 5 (February 2020), p. 5381-5388 |
dc.rights |
open access |
dc.rights |
Tots els drets reservats. |
dc.rights |
https://rightsstatements.org/vocab/InC/1.0/ |
dc.subject |
Self-assembling polypeptides |
dc.subject |
Targeting |
dc.subject |
Nucleolin |
dc.subject |
Triple-negative breast cancer |
dc.subject |
Cancer stem cells |
dc.title |
Engineering a nanostructured nucleolin-binding peptide for intracellular drug delivery in triple-negative breast cancer stem cells |
dc.type |
Article |
dc.description.abstract |
Altres ajuts: La Fundació Marató TV3 (TV32013-133930-1-2), AECC post-doctoral fellowship (AIO14142112SERA) i ICREA Academia award |
dc.description.abstract |
Five peptide ligands of four different cell surface receptors (nucleolin, CXCR1, CMKLR1, and CD44v6) have been evaluated as targeting moieties for triple-negative human breast cancers. Among them, the peptide F3, derived from phage display, promotes the fast and efficient internalization of a genetically fused green fluorescent protein (GFP) inside MDA-MB-231 cancer stem cells in a specific receptor-dependent fashion. The further engineering of this protein into the modular construct F3-RK-GFP-H6 and the subsequent construct F3-RK-PE24-H6 resulted in self-assembling polypeptides that organize as discrete and regular nanoparticles. These materials, 15-20 nm in size, show enhanced nucleolin-dependent cell penetrability. We show that the F3-RK-PE24-H6, based on the Pseudomonas aeruginosa exotoxin A (PE24) as a core functional domain, is highly cytotoxic over target cells. The combination of F3, the cationic peptide (RK), and the toxin domain PE24 in such unusual presentation appears as a promising approach to cell-targeted drug carriers in breast cancers and addresses selective drug delivery in otherwise difficult-to-treat triple-negative breast cancers. |