Title:
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Identification of new biophysical markers for pathological ventricular remodelling in tachycardia-induced dilated cardiomyopathy
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Author:
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Benitez Amaro, Aleyda; Samouillan, Valerie; Jorge, Esther; Dandurand, Jany; Nasarre, Laura; Gonzalo Calvo, David de; Bornachea, Olga; Amorós-Figueras, Gerard; Lacabanne, Colette; Vilades, David; Leta, Rubén; Carreras Costa, Francesc; Gallardo, Alberto; Lerma Puertas, Enrique; Cinca, Juan; Guerra Ramos, José María.; Llorente-Cortés, Vicenta
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Abstract:
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Altres ajuts: This work was co-financed by the European Fund for Regional Development (E.F.R.D.) and Fundacio Marató TV3 (201521 10). |
Abstract:
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Our aim was to identify biophysical biomarkers of ventricular remodelling in tachycardia-induced dilated cardiomyopathy (). Our study includes healthy controls (N = 7) and pigs (N = 10). Molecular analysis showed global myocardial metabolic abnormalities, some of them related to myocardial hibernation in failing hearts, supporting the translationality of our model to study cardiac remodelling in dilated cardiomyopathy. Histological analysis showed unorganized and agglomerated collagen accumulation in the dilated ventricles and a higher percentage of fibrosis in the right () than in the left () ventricle (P = .016). The Fourier Transform Infrared Spectroscopy () 1st and 2nd indicators, which are markers of the myofiber/collagen ratio, were reduced in dilated hearts, with the 1st indicator reduced by 45% and 53% in the and , respectively, and the 2nd indicator reduced by 25% in the . The 3rd indicator, a marker of the carbohydrate/lipid ratio, was up-regulated in the right and left dilated ventricles but to a greater extent in the (2.60-fold vs 1.61-fold, P = .049). Differential scanning calorimetry () showed a depression of the freezable water melting point in ventricles - indicating structural changes in the tissue architecture - and lower protein stability. Our results suggest that the 1st, 2nd and 3rd indicators are useful markers of cardiac remodelling. Moreover, the 2nd and 3rd indicators, which are altered to a greater extent in the right ventricle, are associated with greater fibrosis. |
Subject(s):
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-Biophysical markers -Cardiac remodelling -Collagen -Differential scanning calorimetry -Fourier transform infrared spectroscopy -Heart failure -Myofiber |
Rights:
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open access
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https://creativecommons.org/licenses/by/4.0/ |
Document type:
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Article |
Published by:
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Uri:
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https://ddd.uab.cat/record/227931
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