Título:
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Relationship between the respiratory microbiome and the severity of airflow limitation, history of exacerbations and circulating eosinophils in COPD patients
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Autor/a:
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Millares, Laura; Pascual, Sergi; Montón, Concepción; Garcia-Nuñez, Marian; Lalmolda, Cristina; Faner, Rosa; Casadevall, Carme; Setó, Laia; Capilla, Silvia; Moreno, Amàlia; Castro-Acosta, Ady Angélica; Alvarez-Martinez, Carlos José; Sibila, Oriol; Peces-Barba, Germán; Cosio, Borja G..; Agustí, Àlvar; Gea Guiral, Joaquim; Monsó, Eduard
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Abstract:
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Altres ajuts: This study was funded by Sociedad Española de Neumología y Cirugía Torácica (SEPAR), Laboratorios Menarini S. A, Fundació Pla Armengol and Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES). CIBERES is an initiative of the ISCIII. |
Abstract:
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The respiratory microbiome is altered in COPD patients but its relationship with core components of the disease, such as the severity of airflow limitation, the frequency of exacerbations or the circulating levels of eosinophils, is unclear. Cross-sectional study comprising 72 clinically stable COPD patients (mean age 68 [SD 7.9] years; FEV1 48.7 [SD 20.1]% of reference) who provided spontaneous sputum samples for 16S rRNA gene amplification and sequencing. The microbiome composition was analysed with QIIME. We observed that: (1) more severe airflow limitation was associated with reduced relative abundance (RA) of Treponema and an increase in Pseudomonas ; (2) patients with ≥2 exacerbations the previous year showed a significantly different bacterial community with respect to non-exacerbators (p = 0.014), with changes in 13 genera, including an increase of Pseudomonas, and finally, (3) peripheral eosinophils levels ≥2% were associated with more diverse microbiome [Chao1 224.51 (74.88) vs 277.39 (78.92) p = 0.006; Shannon 3.94 (1.05) vs 4.54 (1.06) p = 0.020], and a significant increase in the RAs of 20 genera. The respiratory microbiome in clinically stable COPD patients varies significantly according to the severity of airflow limitation, previous history of exacerbations and circulating eosinophils levels. The online version of this article (10.1186/s12890-019-0867-x) contains supplementary material, which is available to authorized users. |
Materia(s):
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-Bacterial community -Diversity -Eosinophils -Exacerbations -Sputum -Stable COPD |
Derechos:
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open access
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Tipo de documento:
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Article |
Editor:
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Uri:
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https://ddd.uab.cat/record/226513
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