Títol:
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Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries
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Autor/a:
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Mongui, Álvaro; Pérez Llanos, Francy J.; Yamamoto, Marcio M.; Lozano, Marcela; Zambrano, Maria M.; Portillo, Patricia del; Fernández Becerra, Carmen; Restrepo, Silvia; Portillo Obando, Hernando A. del; Junca, Howard
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Abstract:
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Ajuts: Departamento Administrativo de Ciencias, Tecnología e Innovación (Colciencias), República de Colombia; Convocatoria 489 - 2009, Código 657048925406, Contrato de financiación RC. 427 - 2009 Colciencias - CorpoGen; Programa de Asistencias Graduadas de Universidad de los Andes, Bogotá, Colombia; i Programa Jóvenes Investigadores de Colciencias |
Abstract:
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BACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed |
Matèries:
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-Malaria -Metagenomics -Functional screening -Anti-malarial drugs -Synthetic gene design -Dermaseptin 4 -Bioluminescent Plasmodium falciparum |
Drets:
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open access
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Article |
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Uri:
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https://ddd.uab.cat/record/163154
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