The study of ancient herd-feeding systems is essential to investigate livestock management and the interactions of humans with domestic animals and past environments. This topic for historic periods has recently been investigated through dental microwear analyses. This approach, however, must be used with caution, as it is based on comparisons of established microwear patterns of modern wild animals. Here we present an experimental reference collection of dental microwear for domestic sheep (Ovis aries), created by a controlled-food trial to fill this methodological gap. Fifty sheep were split into five groups of ten, fed with four different types of vegetation potentially used by agropastoral societies (alfalfa, ray-grass, forage, and barley), and administrated following different techniques of processing (wet, dried and fresh). After being fed with a specific and controlled diet, the animals were slaughtered and the microwear patterns on the enamel surface of the lower molars were analysed via standard light stereomicroscopy. The differences found in our experiment between the different diets and processed plants have allowed us to characterize each dietary group and feeding management system. This information is extremely important to correctly interpret the archaeological record.