Title:
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Quantification of gene expression patterns to reveal the origins of abnormal morphogenesis
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Author:
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Martínez Abadías, Neus; Mateu-Estivill, Roger; Sastre-Tomas, Jaume; Motch Perrine, Susan; Yoon, Melissa; Robert Moreno, Alexandre; Swoger, Jim; Russo, Lucia; Kawasaki, Kazuhiko
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Abstract:
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The earliest developmental origins of dysmorphologies are poorly understood in many congenital diseases. They often remain elusive because the first signs of genetic misregulation may initiate as subtle changes in gene expression, which are hard to detect and can be obscured later in development by secondary effects. Here, we develop a method to trace back the origins of phenotypic abnormalities by accurately quantifying the 3D spatial distribution of gene expression domains in developing organs. By applying Geometric Morphometrics to 3D gene expression data obtained by Optical Projection Tomography, we determined that our approach is sensitive enough to find regulatory abnormalities that have never been detected previously. We identified subtle but significant differences in the gene expression of a downstream target of a Fgfr2 mutation associated with Apert syndrome, demonstrating that these mouse models can further our understanding of limb defects in the human condition. Our method can be applied to different organ systems and models to investigate the etiology of malformations. |
Abstract:
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The research leading to these results received funding from the following grants: a European Union Seventh Framework Program (FP7/2007-2013) under grant agreement Marie Curie Fellowship FP7-PEOPLE-2012-IIF 327382, National Institutes of Health grants NICHD P01HD078233 and NIDCR R01DE02298, and a Burroughs-Welcome Fund 2013 Collaborative Research Travel Grant. |
Subject(s):
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-Apert syndrome -Geometric Morphometrics (GM) -Optical Projection Tomography (OPT) -Developmental biology -Developmental defects -Limb development -Mouse -Whole‐mount‐in‐situ hybridization (WMISH) |
Rights:
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© 2018, Martínez-Abadías et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited.
http://creativecommons.org/licenses/by/4.0/ |
Document type:
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Article Article - Published version |
Published by:
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eLife
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