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Título:	Unravelling the encapsulation of DNA and other biomolecules in HAp microcalcifications of human breast cancer tissues by raman imaging
Autor/a:	Marro, Monica; Rodríguez-Rivero, Anna M.; Araujo-Andrade, Cuauhtémoc; Fernández-Figueras, María-Teresa; Pérez-Roca, Laia; Castellà, Eva; Navinés, Jordi; Mariscal, Antonio; Julián, Joan Francesc; Turon, Pau; Loza-Alvarez, Pablo
Notas:	Microcalcifications are detected through mammography screening and, depending on their morphology and distribution (BI-RADS classification), they can be considered one of the first indicators of suspicious cancer lesions. However, the formation of hydroxyapatite (HAp) calcifications and their relationship with malignancy remains unknown. In this work, we report the most detailed three-dimensional biochemical analysis of breast cancer microcalcifications to date, combining 3D Raman spectroscopy imaging and advanced multivariate analysis in order to investigate in depth the molecular composition of HAp calcifications found in 26 breast cancer tissue biopsies. We demonstrate that DNA has been naturally adsorbed and encapsulated inside HAp microcalcifications. Furthermore, we also show the encapsulation of other relevant biomolecules in HAp calcifications, such as lipids, proteins, cytochrome C and polysaccharides. The demonstration of natural DNA biomineralization, particularly in the tumor microenvironment, represents an unprecedented advance in the field, as it can pave the way to understanding the role of HAp in malignant tissues.
Materia(s):	-Microcalcificacions -Mineralització de l’ADN -ADN -Càncer de pulmó -Microambient tumoral -Espectroscòpia -Microcalcificaciones -Mineralización del ADN -ADN -Cáncer de pulmón -Microambiente tumoral -Espectroscopia -Microcalcifications -DNA mineralization -DNA -Lung cancer -Tumor microenvironment -Spectroscopy -61
Derechos:	This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
Tipo de documento:	Artículo Artículo - Versión publicada
Editor:	MDPI
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