dc.contributor
Institut Català de la Salut
dc.contributor
[Sayago C, Sánchez-Wandelmer J, García F] Proteomics Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. [Hurtado B] Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. Cancer Cell Cycle group, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. [Lafarga V] Genomic Instability Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. [Prieto P] Mouse Genome Editing Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Sayago, Cristina
dc.contributor.author
Sánchez-Wandelmer, Jana
dc.contributor.author
Garcia-Martinez, Fernando
dc.contributor.author
Hurtado, Begoña
dc.contributor.author
Lafarga, Vanesa
dc.contributor.author
Prieto, Patricia
dc.date.accessioned
2025-10-25T05:36:31Z
dc.date.available
2025-10-25T05:36:31Z
dc.date.issued
2023-06-19T07:33:12Z
dc.date.issued
2023-06-19T07:33:12Z
dc.date.issued
2023-05-25
dc.identifier
Sayago C, Sánchez-Wandelmer J, García F, Hurtado B, Lafarga V, Prieto P, et al. Decoding protein methylation function with thermal stability analysis. Nat Commun. 2023 May 25;14:3016.
dc.identifier
https://hdl.handle.net/11351/9794
dc.identifier
10.1038/s41467-023-38863-1
dc.identifier.uri
http://hdl.handle.net/11351/9794
dc.description.abstract
Methylation; Proteomics
dc.description.abstract
Metilació; Proteòmica
dc.description.abstract
Metilación; Proteómica
dc.description.abstract
Protein methylation is an important modification beyond epigenetics. However, systems analyses of protein methylation lag behind compared to other modifications. Recently, thermal stability analyses have been developed which provide a proxy of a protein functional status. Here, we show that molecular and functional events closely linked to protein methylation can be revealed by the analysis of thermal stability. Using mouse embryonic stem cells as a model, we show that Prmt5 regulates mRNA binding proteins that are enriched in intrinsically disordered regions and involved in liquid-liquid phase separation mechanisms, including the formation of stress granules. Moreover, we reveal a non-canonical function of Ezh2 in mitotic chromosomes and the perichromosomal layer, and identify Mki67 as a putative Ezh2 substrate. Our approach provides an opportunity to systematically explore protein methylation function and represents a rich resource for understanding its role in pluripotency.
dc.description.abstract
We thank all members of the CNIO Proteomics Unit for discussions, the CNIO Flow Cytometry Unit for flow cytometry support, Cyan Lynch for sharing reagents and Ana Martinez-Val for support with data analysis. This work was supported by SAF2016-74962-R (MINECO) and the European Union Horizon 2020 program INFRAIA project EPIC-XS (project 823839). The CNIO Proteomics Unit belongs to ProteoRed, PRB3- ISCIII, supported by grant PT17/0019/0005. J.M. is supported by the Ikerbasque Programme, Basque Foundation for Science. O.F-C. is supported by grants from the Spanish Ministry of Science, Innovation and Universities (PID2021-128722OB-I00, co-financed with European FEDER funds) and the Spanish Association Against Cancer (AECC; PROYE20101FERN). M.M. lab was supported by grants from MINECO (PID2021-128726 and PDC2022-133408-I00), and Comunidad de Madrid (Y2020/BIO-6519 and S2022/BMD-7437).
dc.format
application/pdf
dc.publisher
Nature Portfolio
dc.relation
Nature Communications;14
dc.relation
https://doi.org/10.1038/s41467-023-38863-1
dc.relation
info:eu-repo/grantAgreement/EC/H2020/823839
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Cèl·lules mare embrionàries
dc.subject
PHENOMENA AND PROCESSES::Genetic Phenomena::Gene Expression Regulation::Epigenesis, Genetic
dc.subject
ANATOMY::Cells::Stem Cells::Pluripotent Stem Cells::Embryonic Stem Cells::Mouse Embryonic Stem Cells
dc.subject
PHENOMENA AND PROCESSES::Chemical Phenomena::Biochemical Phenomena::Alkylation::Methylation
dc.subject
FENÓMENOS Y PROCESOS::fenómenos genéticos::regulación de la expresión génica::epigénesis genética
dc.subject
ANATOMÍA::células::células madre::células madre pluripotentes::células madre embrionarias::células madre embrionarias del ratón
dc.subject
FENÓMENOS Y PROCESOS::fenómenos químicos::fenómenos bioquímicos::alquilación::metilación
dc.title
Decoding protein methylation function with thermal stability analysis
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
