dc.contributor
Institut Català de la Salut
dc.contributor
[Serapiglia V] School of Medicine, Northeast Ohio College of Medicine, Northeast Ohio Medical University, Rootstown Township, OH, United States. [Stephens CA] Department of Psychiatry, University of Illinois at Chicago, Chicago, IL, United States. [Joshi R] Division of Critical Care Medicine, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States. [Aydin E] Department of Pediatric Surgery, Tekirdag Namik Kemal University School of Medicine, Tekirdag, Turkey. Center for Fetal and Placental Research, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, OH, United States. [Oria M, Peiro JL] Center for Fetal and Placental Research, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, OH, United States. Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH, United States. [Marotta M] Laboratori de Bioenginyeria, Teràpia Cel•lular i Cirurgia en Malformacions Congènites, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Varisco BM] Division of Critical Care Medicine, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States. Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH, United States
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Serapiglia, Vincent
dc.contributor.author
Stephens, Chad A.
dc.contributor.author
Joshi, Rashika
dc.contributor.author
Aydin, Emrah
dc.contributor.author
Oria, Marc
dc.contributor.author
Marotta Baleriola, Mario
dc.contributor.author
Peiro, Jose L.
dc.contributor.author
Varisco, Brian
dc.date.accessioned
2025-10-24T08:50:17Z
dc.date.available
2025-10-24T08:50:17Z
dc.date.issued
2022-07-25T09:51:50Z
dc.date.issued
2022-07-25T09:51:50Z
dc.date.issued
2022-02-22
dc.identifier
Serapiglia V, Stephens CA, Joshi R, Aydin E, Oria M, Marotta M, et al. Fetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling. Front Pediatr. 2022 Feb 22;9:780166.
dc.identifier
https://hdl.handle.net/11351/7921
dc.identifier
10.3389/fped.2021.780166
dc.identifier
000769802800001
dc.identifier.uri
http://hdl.handle.net/11351/7921
dc.description.abstract
Basal cell; Fetal tracheal occlusion; Mechanotransduction
dc.description.abstract
Célula basal; Oclusión traqueal fetal; Mecanotransducción
dc.description.abstract
Cèl·lula basal; Oclusió traqueal fetal; Mecanotransducció
dc.description.abstract
Fetal endoscopic tracheal occlusion (FETO) is an emerging surgical therapy for congenital diaphragmatic hernia (CDH). Ovine and rabbit data suggested altered lung epithelial cell populations after tracheal occlusion (TO) with transcriptomic signatures implicating basal cells. To test this hypothesis, we deconvolved mRNA sequencing (mRNA-seq) data and used quantitative image analysis in fetal rabbit lung TO, which had increased basal cells and reduced ciliated cells after TO. In a fetal mouse TO model, flow cytometry showed increased basal cells, and immunohistochemistry demonstrated basal cell extension to subpleural airways. Nuclear Yap, a known regulator of basal cell fate, was increased in TO lung, and Yap ablation on the lung epithelium abrogated TO-mediated basal cell expansion. mRNA-seq of TO lung showed increased activity of downstream Yap genes. Human lung specimens with congenital and fetal tracheal occlusion had clusters of subpleural basal cells that were not present in the control. TO increases lung epithelial cell nuclear Yap, leading to basal cell expansion.
dc.description.abstract
Funding was obtained from NIH/NHLBI R01HL141229 (to BV).
dc.format
application/pdf
dc.publisher
Frontiers Media
dc.relation
Frontiers in Pediatrics;9
dc.relation
https://doi.org/10.3389/fped.2021.780166
dc.rights
Attribution 4.0 International
dc.rights
http://creativecommons.org/licenses/by/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Malalties congènites - Tractament
dc.subject
Hèrnia - Tractament
dc.subject
DISEASES::Congenital, Hereditary, and Neonatal Diseases and Abnormalities::Congenital Abnormalities::Hernias, Diaphragmatic, Congenital
dc.subject
Other subheadings::Other subheadings::Other subheadings::/therapy
dc.subject
ENFERMEDADES::enfermedades y anomalías neonatales congénitas y hereditarias::anomalías congénitas::hernias diafragmáticas congénitas
dc.subject
Otros calificadores::Otros calificadores::Otros calificadores::/terapia
dc.title
Fetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
